Identification of Enterocytozoon bieneusi spores in respiratory samples from an AIDS patient with a 2-year history of intestinal microsporidiosis
Identification of Enterocytozoon bieneusi spores in respiratory samples from an AIDS patient with a 2-year history of intestinal microsporidiosis
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DOI：
10.1128/jcm.35.7.1862-1866.1997
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发表时间：
1997-07-01
影响因子：
9.4
通讯作者：
Pieniazek, NJ
中科院分区：
文献类型：
--
作者：
delAguila, C;LopezVelez, R;Pieniazek, NJ
比氏肠细胞虫（Enterocytozoonbieneusi）是一种微孢子虫，自1985年以来被认为是引起肠道微孢子虫病的病原体，可导致AIDS患者的吸收不良综合征、腹泻和体重减轻。在一位有2年肠道微孢子虫病病史的艾滋病患者的痰、支气管肺泡灌洗液和粪便样本中发现了双孢子虫孢子。通过Weber染色、免疫荧光试验和PCR对孢子进行了表征，通过相同的技术在尿液样品中未检测到微孢子虫。用E. bieneusi-、兔脑炎原虫-、Hellem脑炎原虫-和脑炎原虫（Septata）原虫特异性引物。阿苯达唑和洛哌丁胺治疗导致肠道症状改善，但未根除寄生虫。据我们所知，这是第二次鉴定E.从艾滋病患者获得的呼吸道和肠道样品中的双孢孢子。虽然在这两个病例中均未发现肺病理学改变，但现在清楚的是E。bieneusi能够在呼吸道定植，建议研究人员应注意发现E.呼吸道分泌物中有双孢菌孢子。
Enterocytozoon bieneusi, a microsporidian parasite, has been recognized since 1985 as an agent of intestinal microsporidiosis leading to malabsorption syndrome, diarrhea, and weight loss in AIDS patients, Recently, however, we have identified E. bieneusi spores in the sputum, bronchoalveolar lavage, and stool samples of an AIDS patient with a 2-year history of intestinal microsporidiosis. The spores were characterized by Weber's chromotrope-based staining, immunofluorescence tests, and PCR, No microsporidia were detected in urine samples by the same techniques. PCR was performed with DNAs purified from specimens with E. bieneusi-, Encephalitozoon cuniculi-, Encephalitozoon hellem-, and Encephalitozoon (Septata) intestinalis-specific primers. Treatment with albendazole and loperamide resulted in an improvement of intestinal symptoms, without eradication of the parasite. To our knowledge, this is the second report of the identification of E. bieneusi spores in respiratory and enteric samples obtained from an AIDS patient. Although no pulmonary pathology could be established in either of these cases, it is now clear that E. bieneusi is capable of colonizing the respiratory tract and it is suggested that investigators should be aware of the possibility of finding E. bieneusi spores in respiratory secretions.