SPR and SPR Imaging: Recent Trends in Developing Nanodevices for Detection and Real-Time Monitoring of Biomolecular Events.
SPR and SPR Imaging: Recent Trends in Developing Nanodevices for Detection and Real-Time Monitoring of Biomolecular Events.
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DOI：
10.3390/s16060870
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发表时间：
2016-06-14
期刊：
影响因子：
--
通讯作者：
Bala C
中科院分区：
文献类型：
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作者：
Puiu M;Bala C
在本文中，我们回顾了表面等离子体共振（SPR）技术的基本原理，特别强调了它的优点以及它在区分生物样品的特异性结合响应和干扰效应方面的局限性。虽然SPR传感器已经发展了近30年，但SPR检测还不能减少分析的耗时步骤，而且很难适用于护理点（POC）测试所需的小型化、便携式平台。近场光学的最新进展，导致SPR成像（SPRi）的发展，作为一种强大的光学，无标记的监测工具，用于生物分子事件的多路检测和监测。SPRi芯片的微阵列设计结合了各种金属纳米结构，使得这些光流器件比传统的SPR传感器更适合于诊断和近病人测试。最新的发展表明，SPRi检测是最有前途的基于表面等离子体的技术，满足了在芯片实验室（LOC）技术中实现的需求。
In this paper we review the underlying principles of the surface plasmon resonance (SPR) technique, particularly emphasizing its advantages along with its limitations regarding the ability to discriminate between the specific binding response and the interfering effects from biological samples. While SPR sensors were developed almost three decades, SPR detection is not yet able to reduce the time-consuming steps of the analysis, and is hardly amenable for miniaturized, portable platforms required in point-of-care (POC) testing. Recent advances in near-field optics have emerged, resulting in the development of SPR imaging (SPRi) as a powerful optical, label-free monitoring tool for multiplexed detection and monitoring of biomolecular events. The microarrays design of the SPRi chips incorporating various metallic nanostructures make these optofluidic devices more suitable for diagnosis and near-patient testing than the traditional SPR sensors. The latest developments indicate SPRi detection as being the most promising surface plasmon-based technique fulfilling the demands for implementation in lab-on-a-chip (LOC) technologies.