Induction of Stat3, a signal transducer and transcription factor, in reactive microglia following transient focal cerebral ischaemia
Induction of Stat3, a signal transducer and transcription factor, in reactive microglia following transient focal cerebral ischaemia
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DOI：
10.1111/j.1460-9568.1996.tb01556.x
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发表时间：
1996-12-01
影响因子：
3.4
通讯作者：
Ferrer, I
中科院分区：
文献类型：
--
作者：
Planas, AM;Soriano, MA;Ferrer, I
Stat3是细胞质信号转导子和转录激活子家族的成员，在生理条件下在体内大鼠脑中发现，并在短暂局灶性脑缺血后受到刺激。大脑中动脉闭塞的短暂发作在大脑中动脉闭塞后4天经历神经细胞死亡的区域诱导强烈的小胶质细胞反应。这是伴随着Stat3在同侧皮质和纹状体的表达增加，所揭示的组织提取物的蛋白质印迹。免疫组化显示Stat3在脑缺血后4、7和15天的反应性小胶质细胞中强烈诱导。Stat3表达于小胶质细胞胞质内，但在许多小胶质细胞中，Stat3免疫反应性也分布于细胞核内。这些结果表明，Stat3介导的信号转导和激活转录反应性小胶质细胞在体内脑缺血后。
Stat3, a member of the family of cytoplasmic signal transducers and activators of transcription, was found in the rat brain in vivo under physiological conditions and was stimulated following transient focal cerebral ischaemia. A transient episode of middle cerebral artery occlusion induced a strong microglial response in the areas undergoing neural cell death from 4 days after middle cerebral artery occlusion. This was accompanied by increased expression of Stat3 in the ipsilateral cortex and striatum, as revealed by Western blotting of tissue extracts. Immunohistochemistry showed strong induction of Stat3 in reactive microglial cells 4, 7 and 15 days after cerebral ischaemia. Stat3 was seen in the microglia cytoplasm, but in many microglial cells immunoreactivity was also distributed within the nucleus. These results suggest that Stat3 mediates signal transduction and activates transcription in reactive microglia in vivo following brain ischaemia.