Efficient chemiluminescent ZnO nanoparticles for cellular imaging
Efficient chemiluminescent ZnO nanoparticles for cellular imaging
复制标题
用于细胞成像的高效化学发光氧化锌纳米颗粒
DOI：
10.1016/j.jlumin.2020.117111
复制
发表时间：
2020-05-01
影响因子：
3.6
通讯作者：
Shan, Chong-Xin
中科院分区：
文献类型：
--
作者：
Liu, Zhi-Yu;Shen, Cheng-Long;Shan, Chong-Xin
过氧草酸盐燃料和过氧化氢诱导的化学发光在化学检测和生物成像中得到了广泛的应用。然而，化学发光体系中的化学发光体大多集中在有机小分子染料上，由于其潜在的生物毒性和较低的光稳定性，在实际应用中遇到了严重的障碍。在过氧草酸盐和过氧化氢溶液中加入具有黄色光致发光(PL)的生物相容的氧化锌纳米颗粒(氧化锌纳米颗粒)，得到明亮的蓝色化学发光，这是由于纳米氧化锌颗粒的锌间隙与化学发光过程中产生的高能量中间体相互作用而产生的。在硅烷偶联剂的作用下，氧化锌纳米粒子的化学发光量子产率从6.2×10~(-6)提高到无机纳米粒子的3.7×10~(-4)E.mol(-1)，这是由于氧化硅壳层的保护作用。由于具有良好的化学发光性能，纳米氧化锌首次被用作生物成像的化学发光探针，为未来生物传感器或生物成像的设计开辟了一条新的途径。
Chemiluminescence (CL) induced by peroxalate fuel and hydrogen peroxide has been widely applied in chemical detection and bioimaging. Nevertheless, the chemiluminescent emitters in the CL are focused on organic small-molecular dyes, most of which encounter serious obstacles in practical application due to their potential biotoxicity and low photostability. Here, bright blue CL is observed when biocompatible zinc oxide nanoparticles (ZnO NPs) with yellow photoluminescence (PL) are added into peroxalate and hydrogen peroxide solution, which is originated from the interaction between the zinc interstitial of ZnO NPs and the energy-rich intermediate produced in the CL process. With the modification of silane coupler, the CL quantum yield of ZnO NPs presents two orders of magnitude increase from 6.2 x 10(-6) to record 3.7 x 10(-4) E.mol(-1) for inorganic NPs owing to the protective effect from silicon oxide shell layer. By virtual of excellent CL ability, ZnO NPs have been firstly used as CL probes in bioimaging, which opens the way to a new design freedom for biosensor or bioimaging in the future.