Colocalization of GABA, glycine, and their receptors at synapses in the rat spinal cord
Colocalization of GABA, glycine, and their receptors at synapses in the rat spinal cord
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DOI：
10.1523/jneurosci.16-03-00974.1996
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发表时间：
1996-02
影响因子：
5.8
通讯作者：
A. Todd;C. Watt;R. Spike;W. Sieghart
中科院分区：
文献类型：
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作者：
A. Todd;C. Watt;R. Spike;W. Sieghart
为了确定GABA和甘氨酸是否可以作为在大鼠脊髓突触的共递质，我们比较了超微结构分布的GABAA受体β 3亚基的甘氨酸受体相关蛋白gephyrin和结合这与植入后检测GABA和甘氨酸。我们还使用了双重免疫荧光方法来证实桥蛋白与整个脊髓中的甘氨酸受体α 1亚基相关。GABAA β 3-亚单位免疫反应主要局限于突触，并且在大多数这些突触中，突触前轴突是GABA-免疫反应性的。许多突触表现出GABAA β 3和桥蛋白的免疫反应性，并且在大多数这些突触中，GABA和甘氨酸在突触前轴突中富集。这些结果有力地支持了GABA和甘氨酸在脊髓中发生共传递的观点。
To determine whether GABA and glycine can act as cotransmitters at synapses in the rat spinal cord, we have compared the ultrastructural distribution of GABAA-receptor beta 3 subunit with that of the glycine receptor-associated protein gephyrin and combined this with postembedding detection of GABA and glycine. We also used a dual- immunofluorescence method to confirm that gephyrin was associated with the glycine-receptor alpha 1 subunit throughout the cord. GABAA beta 3- subunit immunoreactivity was restricted primarily to synapses, and at a majority of these synapses the presynaptic axon was GABA- immunoreactive. Many synapses showed both GABAA beta 3 and gephyrin immunoreactivity, and at most of these synapses GABA and glycine were enriched in the presynaptic axon. These results strongly support the idea that cotransmission by GABA and glycine occurs in the spinal cord.