pDNA/poly(L-lysine) Polyplexes Functionalized with a pH-Sensitive Charge-Conversional Poly(aspartamide) Derivative for Controlled Gene Delivery to Human Umbilical Vein Endothelial Cells.
pDNA/poly(L-lysine) Polyplexes Functionalized with a pH-Sensitive Charge-Conversional Poly(aspartamide) Derivative for Controlled Gene Delivery to Human Umbilical Vein Endothelial Cells.
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DOI：
10.1002/marc.201000056
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发表时间：
2010-07
影响因子：
4.6
通讯作者：
Mai Sanjoh;Shigehiro Hiki;Yan Lee;M. Oba;K. Miyata;Takehiko Ishii;K. Kataoka
中科院分区：
文献类型：
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作者：
Mai Sanjoh;Shigehiro Hiki;Yan Lee;M. Oba;K. Miyata;Takehiko Ishii;K. Kataoka
为了提高质粒DNA（pDNA）与多聚赖氨酸（PLys）的复合物的基因转染效率，将内体逃逸功能整合到pDNA中。CCP对内体pH值具有电荷转换功能，导致pDNA/PLys复合物释放到细胞质中。细胞摄取完整的CCP整合的三元复合物，其对人脐静脉内皮细胞（HUVECs）表现出比pDNA/PLys复合物明显更高的转染效率和更低的细胞毒性。这与用共聚焦激光扫描显微镜直接观察到的与pDNA/PLys复合物相比CCP整合的三元复合物的促进的内体逃逸一致。
An efficient endosome-escaping function was integrated into the polyplex of plasmid DNA (pDNA) with poly(L-lysine) (PLys) to improve its gene transfection efficiency through electrostatic coating with charge-conversional polymer (CCP). CCP showed charge-conversional function responding to endosomal pH, leading to the release of pDNA/PLys polyplex into the cytoplasm. The cells took up the intact CCP-integrated ternary polyplex, which exerted appreciably higher transfection efficiency with lower cytotoxicity than pDNA/PLys polyplex against human umbilical vein endothelial cells (HUVECs). This is consistent with the facilitated endosomal escape of the CCP-integrated ternary polyplex compared to the pDNA/PLys polyplex as directly observed with confocal laser-scanning microscopy.