TRANSFORMING GROWTH-FACTOR BETA-1-MEDIATED INHIBITION OF SMOOTH-MUSCLE CELL-PROLIFERATION IS ASSOCIATED WITH A LATE G1 CELL-CYCLE ARREST
TRANSFORMING GROWTH-FACTOR BETA-1-MEDIATED INHIBITION OF SMOOTH-MUSCLE CELL-PROLIFERATION IS ASSOCIATED WITH A LATE G1 CELL-CYCLE ARREST
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DOI：
10.1002/jcp.1041560108
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发表时间：
1993-07-01
影响因子：
5.6
通讯作者：
HOWE, PH
中科院分区：
文献类型：
--
作者：
REDDY, KB;HOWE, PH
观察了转化生长因子β 1（TGF β 1）对体外培养的主动脉平滑肌细胞（SMC）增殖反应的影响。TGF β 1显著抑制人和牛SMC的生长。TGF β 1抑制快速生长的SMC和静止血清刺激的SMC，其ID 50约为0.5 ng/ml，并且在10 ng/ml TGF β 1时观察到最大抑制。在TGF β 1存在下，静止血清刺激的SMC进展到细胞周期的G1期，但在时间上位于S期1-2小时的点处可逆地停滞。从这个晚期G1 TGF β 1阻滞点释放导致在2小时内进入S期。与这种抑制作用相关的是p34 cdc 2蛋白激酶的组蛋白H1激酶活性降低，而TGF β 1对p34 cdc 2的转录或翻译没有影响。在这些生长抑制条件下，TGF β 1仍然能够上调纤连蛋白mRNA的表达。这些结果表明，TGF β 1在SMC中的生长抑制与G1晚期p34 cdc 2活性的调节有关。(C)1993 Wiley-Liss，Inc.
The effect of transforming growth factor beta1 (TGFbeta1) on the proliferative response of aortic smooth muscle cells (SMC) in vitro was investigated. TGFbeta1 substantially inhibited the growth of human and bovine SMC. Rapidly growing SMC and quiescent serum-stimulated SMC were inhibited by TGFbeta1 with an ID50 of approximately 0.5 ng/ml and maximal inhibition was observed at 10 ng/ml TGFbeta1 In the presence of TGFbeta1, quiescent serum-stimulated SMC progress into the G1 phase of the cell cycle, but become reversibly arrested at a point temporally located 1-2 hours from S phase. Release from this late G1 TGFbeta1 arrest point results in S phase entry within 2 hours. Associated with this inhibitory effect is a decrease in the histone H1 kinase activity of p34cdc2 protein kinase while TGFbeta1 has no effect on the transcription or translation of p34cdc2. Under these growth inhibitory conditions, TGFbeta1 is still capable of upregulating the expression of fibronectin mRNA. These results suggest that TGFbeta1 growth inhibition in SMC is associated with the regulation of p34cdc2 activity in late G1. (C) 1993 Wiley-Liss, Inc.