Altered density, metabolism and surface receptors of eosinophils in eosinophilia.
Altered density, metabolism and surface receptors of eosinophils in eosinophilia.
复制标题
嗜酸性粒细胞增多症中嗜酸性粒细胞的密度、代谢和表面受体发生改变。
作者：
I. Winqvist;T. Olofsson;I. Olsson;A. Persson;T. Hallberg
对正常人和嗜酸性粒细胞增多症患者的嗜酸性粒细胞进行了比较。通过Percoll密度梯度离心获得高度纯化的嗜酸性粒细胞。研究了密度分布，氧气消耗后，坚持血清包被的Sephadex和细胞表面受体的IgG和补体的表达。在嗜酸性粒细胞白血病中，嗜酸性粒细胞的密度异常低。在高嗜酸性粒细胞综合征（HES）中也检测到血嗜酸性粒细胞的异常光密度分数。HES的轻密度嗜酸性粒细胞显示脱颗粒的形态学体征，与颗粒嗜酸性粒细胞阳离子蛋白（ECP）含量低的发现一致，表明循环中的脱颗粒或骨髓中的异常颗粒形成。此外，这些细胞表现出更高的耗氧量比正常密度的嗜酸性粒细胞粘附血清包被的葡聚糖凝胶。低密度的嗜酸性粒细胞比高密度的细胞表现出更多的细胞与Fc-IgG和补体受体。同样，渗出液中的嗜酸性粒细胞显示出异常低的密度，其耗氧量高于正常值，表明组织中的嗜酸性粒细胞可能被激活。在一例HES患者中，发热事件导致正常密度的嗜酸性粒细胞消失，而异常低密度嗜酸性粒细胞增加。我们的研究结果表明，嗜酸性粒细胞增多症患者的嗜酸性粒细胞可能是'激活'的循环。
A comparison was made between eosinophils from normal persons and patients with eosinophilia. Highly purified eosinophils were obtained by centrifugation in a Percoll density gradient. Studies were carried out on density distribution, oxygen consumption upon adherence to serum-coated Sephadex and expression of cell surface receptors for IgG and complement. In eosinophil leukaemia the density of eosinophils was abnormally low. Abnormal light density fractions of blood eosinophils were also detected in the hypereosinophilic syndrome (HES). Light density eosinophils of HES showed morphological signs of degranulation consonant with the finding of a low content of granular eosinophil cationic protein (ECP) suggesting degranulation in the circulation or abnormal granule formation in the marrow. In addition, such cells exhibited a higher oxygen consumption than eosinophils with normal density upon adherence to serum-coated Sephadex. Low density eosinophils showed a greater number of cells with Fc-IgG and complement receptors than high density cells. Likewise exudate eosinophils displayed an abnormally low density with higher than normal oxygen consumption indicating that eosinophils may be activated in the tissues. In one patient with HES, a febrile episode resulted in a disappearance of eosinophils with a normal density while abnormal low density eosinophils increased. Our findings suggest that eosinophils from some patients with eosinophilia may be 'activated' in the circulation.