Fifteen years of the Protein Crystallography Station: the coming of age of macromolecular neutron crystallography.
Fifteen years of the Protein Crystallography Station: the coming of age of macromolecular neutron crystallography.
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DOI：
10.1107/s205225251601664x
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发表时间：
2017-01-01
期刊：
影响因子：
3.9
通讯作者：
Unkefer CJ
中科院分区：
文献类型：
--
作者：
Chen JC;Unkefer CJ
这篇文章重点介绍了洛斯阿拉莫斯蛋白质晶体学站的科学和技术贡献，这是第一个专门建造的大分子晶体学站。位于洛斯阿拉莫斯中子散射中心（LANSCE）的蛋白质晶体学站（PCS）是第一条在空间中子源上建造的大分子晶体学光束线。在测试和调试之后，PCS用户程序由能源部科学办公室（DOE-OBER）的生物和环境研究计划资助了13年（2002-2014）。PCS一直是北美唯一的专用大分子中子晶体学站，直到2012年橡树岭国家实验室的MaNDi和IMAGINE仪器开始建造和调试。该仪器产生了许多对该领域做出贡献的研究和技术成果，清楚地证明了中子晶体学在帮助科学家了解酶反应机制、氢键和氢原子位置可视化方面的作用，这些对几乎所有化学反应都至关重要。在此期间，中子晶体学成为一种越来越受到关注的技术，并通过PCS研究人员领导的软件开发变得更加集成到大分子晶体学中。这篇综述强调了PCS对大分子中子晶体学的贡献，并概述了中子晶体学的历史和大分子中子晶体学从20世纪60年代到90年代的发展。
This article highlights scientific and technical contributions from the Protein Crystallography Station at Los Alamos, the first purpose-built macromolecular crystallography station at a spallation neutron source. The Protein Crystallography Station (PCS), located at the Los Alamos Neutron Scattering Center (LANSCE), was the first macromolecular crystallography beamline to be built at a spallation neutron source. Following testing and commissioning, the PCS user program was funded by the Biology and Environmental Research program of the Department of Energy Office of Science (DOE-OBER) for 13 years (2002–2014). The PCS remained the only dedicated macromolecular neutron crystallography station in North America until the construction and commissioning of the MaNDi and IMAGINE instruments at Oak Ridge National Laboratory, which started in 2012. The instrument produced a number of research and technical outcomes that have contributed to the field, clearly demonstrating the power of neutron crystallography in helping scientists to understand enzyme reaction mechanisms, hydrogen bonding and visualization of H-atom positions, which are critical to nearly all chemical reactions. During this period, neutron crystallography became a technique that increasingly gained traction, and became more integrated into macromolecular crystallography through software developments led by investigators at the PCS. This review highlights the contributions of the PCS to macromolecular neutron crystallography, and gives an overview of the history of neutron crystallography and the development of macromolecular neutron crystallography from the 1960s to the 1990s and onwards through the 2000s.