Surface Charge-Dependent Toxicity of Silver Nanoparticles
Surface Charge-Dependent Toxicity of Silver Nanoparticles
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DOI：
10.1021/es1034188
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发表时间：
2011-01-01
影响因子：
11.4
通讯作者：
Tolaymat, Thabet M.
中科院分区：
文献类型：
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作者：
El Badawy, Amro M.;Silva, Rendahandi G.;Tolaymat, Thabet M.
由于银纳米颗粒（AgNPs）的广泛应用，其一旦释放到环境中的潜在影响令人担忧。据报道，AgNPs的毒性取决于多种因素，如粒径、形状和封盖剂，尽管这些因素可能在AgNPs的毒性中起作用，但本文的结果表明，表面电荷是控制AgNPs毒性的最重要因素之一。在目前的研究中，研究了四种AgNPs的毒性，这些AgNPs代表了从高负电荷到高正电荷的不同表面充电情况。这些AgNPs是(1)未包覆的H-2-AgNPs，(2)柠檬酸盐包覆的AgNPs (citrate -AgNPs),(3)聚乙烯吡啶酮包覆的AgNPs (PVP-AgNPs)，以及(4)支链聚乙烯亚胺包覆的AgNPs （BPEI-AgNPs）。我们的研究结果清楚地表明，AgNPs对所研究的芽孢杆菌具有表面电荷依赖的毒性。此外，在引入微生物之前，利用超滤膜纯化AgNPs悬浮液中的残留杂质。这一步对于确定AgNPs的真正毒性至关重要，在大多数毒性研究报告中要么缺失，要么没有明确提及。
As a result of the extensive number of applications of silver nanoparticles (AgNPs), their potential impacts, once released into the environment, are of concern. The toxicity of AgNPs was reported to be dependent on various factors such as particle size, shape and capping agent Although these factors may play a role in AgNPs toxicity, the results presented herein suggest that surface charge is one of the most important factors that govern the toxicity of AgNPs. In the current study, the toxicity of four AgNPs representing various surface charging scenarios ranging from highly negative to highly positive was investigated. These AgNPs were (1) uncoated H-2-AgNPs, (2) citrate coated AgNPs (Citrate-AgNPs), (3) polyvinylpyrrolidone coated AgNPs (PVP-AgNPs), and (4) branched polyethyleneimine coated AgNPs (BPEI-AgNPs). Our results clearly demonstrate that the AgNPs exhibited surface charge-dependent toxicity on the bacillus species investigated. Furthermore, ultrafiltration membranes were utilized to purify the AgNPs suspensions from residual impurities prior to the introduction to the microbes. This step was crucial in determining the true AgNPs toxicity and is either missing or not explicitly mentioned in most of the reported toxicity studies.