Ultrasound-Induced Escherichia coli O157:H7 Cell Death Exhibits Physical Disruption and Biochemical Apoptosis.
Ultrasound-Induced Escherichia coli O157:H7 Cell Death Exhibits Physical Disruption and Biochemical Apoptosis.
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DOI：
10.3389/fmicb.2018.02486
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发表时间：
2018
影响因子：
5.2
通讯作者：
Ding T
中科院分区：
文献类型：
--
作者：
Li J;Ma L;Liao X;Liu D;Lu X;Chen S;Ye X;Ding T
超声作为一种物理加工和保存工具的潜在用途引起了工业界和科学界的极大兴趣。本研究以大肠杆菌O157:H7为模型微生物，研究超声诱导的细胞死亡。低强度超声诱导的膜电位和膜间离子交换的微小变化增加了大肠杆菌O157:H7的膜通透性，这种可逆的亚致死效应可以保持大肠杆菌O157:H7的活力，同时有利于生物加工应用。而高强度超声对大肠杆菌O157:H7具有不可逆的致死作用，可应用于微生物灭活领域。此外，低强度和高强度超声均可诱导大肠杆菌O157:H7的物理破坏或触发基因编码的凋亡。在大肠杆菌O157:H7中，活性氧的积累和三磷酸腺苷的减少可能与凋亡的生理生化标志有关，包括暴露的磷脂酰丝氨酸和活化的半胱天冬酶。该结果为非热物理处理对细菌失活的机制提供了新的认识，为进一步研究凋亡细菌的细胞信号传导和代谢途径奠定了基础。
Ultrasound has attracted great interest of both industry and scientific communities for its potential use as a physical processing and preservation tool. In this study, Escherichia coli O157:H7 was selected as the model microbe to investigate the ultrasound-induced cell death. Slight variations in membrane potential and ion exchanges across membrane induced by low-intensity ultrasound increased the membrane permeability of E. coli O157:H7, and this reversible sublethal effect can preserve the viability of E. coli O157:H7 and meanwhile be beneficial for bioprocessing application. In comparison, high-intensity ultrasound resulted in irreversible lethal effect on E. coli O157:H7, which can be applied in the field of microbial inactivation. In addition, both low- and high-intensity ultrasound induced either physical destruction or trigger genetically encoded apoptosis of E. coli O157:H7. Accumulation of reactive oxygen species and decrease of adenosine tri-phosphate might be related to the physiological and biochemical hallmarks of apoptosis, including exposed phosphatidylserine and activated caspases in E. coli O157:H7. The result provides novel insight into the mechanisms of non-thermal physical treatment on the inactivation of bacteria and lays foundation for the further research on the cell signaling and metabolic pathway in apoptotic bacteria.
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