Neurochemical characterization of the cerebellar-recipient motor thalamic territory in the macaque monkey
Neurochemical characterization of the cerebellar-recipient motor thalamic territory in the macaque monkey
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DOI：
10.1111/j.1460-9568.2005.04020.x
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发表时间：
2005-04-01
影响因子：
3.4
通讯作者：
Luppino, G
中科院分区：
文献类型：
--
作者：
Calzavara, R;Zappalà, A;Luppino, G
对猕猴运动丘脑的免疫结构进行了分析，以寻找丘脑区域可能的神经化学特征，这些特征在细胞结构上无法定义，与不同的功能途径相关。对 15 只猕猴的丘脑切片进行处理，以可视化钙结合蛋白 (CB)、小白蛋白 (PV)、钙视网膜蛋白 (CR) 和 SMI-32 免疫反应性 (ir)。 PV-、CR-和SMI-32ir 分布与已知的功能细分没有显示出任何明显的相关性。相反，CBir 分布可靠地定义了两个明显不同的运动丘脑区域，一个以高细胞和神经纤维 CBir（CB 阳性区域）为特征，另一个以非常低的细胞和神经纤维 CBir（CB 阴性区域）为特征。这两个神经化学上不同的区室，CB 阴性和 CB 阳性区域，似乎分别对应于小脑和基底神经节受体区域。为了验证 CB 阴性区域与运动丘脑小脑接收区的可能对应关系，我们比较了两只猴子的小脑丘脑投射的分布与 CBir 的分布。小脑传入末梢的分布与之前的报告相似，并且符合运动丘脑中小脑接受区不遵守细胞结构边界的概念。与 CB 免疫结构的比较显示，运动丘脑中顺行标记的分布与 CB 阴性区域之间非常密切的对应关系，表明 CB 阴性区域代表小脑受体区域的结构对应物。因此，CB 免疫染色可能是描述丘脑皮质投射与基底神经节或小脑环之间的关联以及建立非人类灵长类动物和人类运动丘脑之间可能的同源性的有用工具。
The immunoarchitectonics of the macaque motor thalamus was analysed to look for a possible neurochemical characterization of thalamic territories, which were not definable cytoarchitectonically, associated with different functional pathways. Thalamic sections from 15 macaque monkeys were processed for visualization of calbindin (CB), parvalbumin (PV), calretinin (CR) and SMI-32 immunoreactivity (ir). PV-, CR- and SMI-32ir distributions did not show any clear correlation with known functional subdivisions. In contrast, CBir distribution reliably defined two markedly distinct motor thalamic territories, one characterized by high cell and neuropil CBir (CB-positive territory), the other by very low cell and neuropil CBir (CB-negative territory). These two neurochemically distinct compartments, the CB-negative and the CB-positive territories, appear to correspond to the cerebellar- and basal ganglia-recipient territories, respectively. To verify the possible correspondence of the CB-negative territory with the cerebellar-recipient sector of the motor thalamus, we compared the distribution of cerebello-thalamic projections with the distribution of CBir in two monkeys. The distribution of cerebellar afferent terminals was similar to that reported from previous reports and in line with the notion that in the motor thalamus the cerebellar-recipient territory does not respect cytoarchitectonic boundaries. Comparison with CB immunoarchitecture showed very close correspondence in the motor thalamus between the distribution of the anterograde labeling and the CB-negative territory, suggesting that the CB-negative territory represents the architectonic counterpart of the cerebellar-recipient territory. CB immunostaining may therefore represent a helpful tool for describing the association between thalamocortical projections and the basal ganglia or the cerebellar loops and for establishing possible homologies between the motor thalamus of non-human primates and humans.