Cloning and characterization of a novel Kruppel-like zinc finger gene, ZNF268, expressed in early human embryo
Cloning and characterization of a novel Kruppel-like zinc finger gene, ZNF268, expressed in early human embryo
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DOI：
10.1016/s0167-4781(01)00194-4
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发表时间：
2001-04-16
期刊：
影响因子：
--
通讯作者：
Li, WX
中科院分区：
文献类型：
--
作者：
Gou, DM;Sun, Y;Li, WX
目的是识别与人类早期胚胎发育有关的基因。我们利用差异杂交技术从3周龄的人胚胎cDNA文库中克隆了一个新的人锌指基因ZNF268。ZNF268的完整cDNA序列由2841个核苷酸组成，编码一个947个氨基酸的蛋白质，其N端有一个Kruppel相关框(KRAB)结构域，C端有24个锌指。Northern杂交分析表明，ZNF268基因主要在3-5周龄的人胚胎中表达，提示其在胚胎发育中可能起到一定的作用。该基因由六个外显子组成，跨越约22kb的基因组DNA。根据HTGS数据库中的基因组序列。ZNF268基因被分配给人类5号染色体。(C)2001爱思唯尔科学公司保留所有权利。
With the aim of identifying genes involved in early human embryonic development. we have isolated a cDNA clone representing a novel human zinc finger gene ZNF268 from 3 week old human embryo cDNA library using a differential hybridization strategy. The complete cDNA sequence of ZNF268 contained an open reading frame of 2841 nucleotides that encodes a 947 amino acid protein with an N-terminal Kruppel-associated box (KRAB) domain and 24 C-terminal zinc fingers. Northern blot analysis showed that ZNF268 mRNA is mainly expressed in 3-5 week old human embryos suggesting it could play certain roles in the embryogenesis. The gene consists of six exons spanning about 22 kb of genomic DNA. According to the genomic sequence from the HTGS database. the ZNF268 gene is assigned to human chromosome 5. (C) 2001 Elsevier Science B.V. All rights reserved.