Potential for aflatoxin B1 and B2 production by Aspergillus flavus strains isolated from rice samples
Potential for aflatoxin B1 and B2 production by Aspergillus flavus strains isolated from rice samples
复制标题
从大米样品中分离出的黄曲霉菌株生产黄曲霉毒素 B1 和 B2 的潜力
DOI：
10.1016/j.sjbs.2014.09.013
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发表时间：
2015-03-01
影响因子：
4.4
通讯作者：
Liu, Chenglan
中科院分区：
文献类型：
--
作者：
Lai, Xianwen;Zhang, He;Liu, Chenglan
在这项研究中，我们研究了从中国收集的127株黄曲霉菌株在稻米中产生黄曲霉毒素B-1（AF B（1））和B-2（AF B（2））的潜力。将这些菌株接种到水稻谷粒上，并在28 ℃下孵育21天。提取黄曲霉毒素B（1）和黄曲霉毒素B（2），采用高效液相色谱-荧光检测法进行定量。在被测菌株中，37%的菌株产生AFB（1）和AFB（2），AFB（1）的水平范围为175至124101 μ g kg（-1），AFB（2）的水平范围为未检出至10329 μ g kg（-1）。AFB（1）和AFB（2）的平均产量分别为5884 μ g kg（-1）和1968 μ g kg（-1）。总体而言，大多数产黄曲霉毒素菌株在水稻中产生的AFB（1）水平高于AFB（2）。所得资料可用作评估食米样本受黄曲霉毒素污染的风险。(C)2014年沙特国王大学。Elsevier B. V.制作和主持。保留所有权利。
In this study, we investigated the potential for aflatoxin B-1 (AFB(1)) and B-2 (AFB(2)) production in rice grain by 127 strains of Aspergillus flavus isolated from rice grains collected from China. These strains were inoculated onto rice grains and incubated at 28 degrees C for 21 days. AFB(1) and AFB(2) were extracted and quantified by high-performance liquid chromatography coupled with fluorescence detection. Among the tested strains, 37% produced AFB(1) and AFB(2) with levels ranging from 175 to 124101 mu g kg(-1) for AFB(1) and from not detected to 10329 mu g kg(-1) for AFB(2). The mean yields of these isolates were 5884 mu g kg(-1) for AFB(1) and 1968 mu g kg(-1) for AFB(2). Overall, most of the aflatoxigenic strains produced higher levels of AFB(1) than AFB(2) in rice. The obtained information is useful for assessing the risk of aflatoxin contamination in rice samples. (C) 2014 King Saud University. Production and hosting by Elsevier B.V. All rights reserved.