Purification of a recombinant protein with cellulose-binding module 3 as the affinity tag.
Purification of a recombinant protein with cellulose-binding module 3 as the affinity tag.
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DOI：
10.1007/978-1-4939-1034-2_3
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发表时间：
2014
影响因子：
--
通讯作者：
Dongmei Wang;Jiong Hong
中科院分区：
文献类型：
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作者：
Dongmei Wang;Jiong Hong
在生物经济中发挥重要作用的常用酶的大规模生产中，对易于操作和低成本的蛋白质纯化方法有很高的需求。以CBM3（家族3纤维素结合模块）为亲和标签，开发了一种低成本、快速的重组蛋白纯化系统。本方案描述了以CBM3作为亲和标签在毕赤酵母中表达的CBM3融合蛋白和无标签蛋白的纯化。
Easy-to-perform and low-cost protein purification methods are in high demand for the mass production of commonly used enzymes that play an important role in bioeconomy. A low-cost and rapid recombinant protein purification system was developed using CBM3 (family 3 cellulose-binding module) as affinity tag. This protocol describes the purification of CBM3-fusion protein and tag-free protein expressed inPichia pastorisusing CBM3 as an affinity tag.