In vitro antiplasmodial activity of ethanolic extracts of seaweed macroalgae against Plasmodium falciparum
In vitro antiplasmodial activity of ethanolic extracts of seaweed macroalgae against Plasmodium falciparum
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DOI：
10.1007/s00436-010-2185-3
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发表时间：
2011-06-01
影响因子：
2
通讯作者：
Venkatesan, Malaiyandi
中科院分区：
文献类型：
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作者：
Ravikumar, Sundaram;Inbaneson, Samuel Jacob;Venkatesan, Malaiyandi
疟疾是许多发展中国家的主要健康问题。抗药性的恶性疟原虫引起人类中最致命的疟疾形式，并且它被描述为引起发病率和死亡率增加的公共卫生灾难。从Mandapam沿海地区采集了属于4个不同科（Rhodomelaceae、Cladophoraceae、Ulvaceae和Caulerpaceae）的13种海藻，并对海藻提取物进行了体外抗恶性疟原虫活性测试。其中，蕨藻提取物（IC（50）5.06 μ g中心点ml（-1））比其它海藻提取物显示出潜在的抗疟原虫活性，并且它可以与阳性对照蒿甲醚（IC（50）4.09 μ g中心点ml（-1））相当。盾叶蕨藻（Caulerpapeltata）的IC（50）为16.69 μ g中心点ml（-1），其IC（50）值低于阳性对照氯喹（IC（50）为19.59 μ g中心点ml（-1））。统计学分析表明，在体外抗疟原虫活性的浓度和时间之间观察到显着的（P < 0.05）。还进行了对红细胞的化学损伤，结果表明，在孵育48小时后，海藻提取物的乙醇提取物对红细胞没有形态学变化。体外抗疟原虫活性可能是由于海藻乙醇提取物中存在糖、蛋白质和酚类物质。结果表明，海藻醇提物具有较好的抗氧化活性。Escherichia和C. peltata拥有开发抗疟原虫药物的先导化合物。
Malaria is a major health problem in many developing countries. The drugs resistant Plasmodium falciparum causes the most virulent form of malaria in humans and it is described as a public health disaster causing increased morbidity and mortality. Thirteen seaweeds species which belong to four different families (Rhodomelaceae, Cladophoraceae, Ulvaceae, and Caulerpaceae) were collected from Mandapam coastal area and the seaweeds extracts were tested for in vitro antiplasmodial activity against P. falciparum. Among them, Caulerpa toxifolia (IC(50) 5.06 mu g center dot ml(-1)) showed potential antiplasmodial activity than other seaweeds extracts and it can be comparable with the positive control artemether (IC(50) 4.09 mu g center dot ml(-1)). Caulerpa peltata (IC(50) 16.69 mu g center dot ml(-1)) also exhibited good antiplasmodial activity and the IC(50) value is lesser than the positive control chloroquine (IC(50) 19.59 mu g center dot ml(-1)). Statistical analysis reveals that significant in vitro antiplasmodial activity (P < 0.05) was observed between the concentrations and time of exposure. The chemical injury to erythrocytes was also carried out and it shows that no morphological changes in erythrocytes by the ethanolic extract of seaweeds extracts after 48 h of incubation. The in vitro antiplasmodial activity might be due to the presence of sugars, proteins, and phenols in the ethanolic extracts of seaweeds. It is concluded from the present study that, the ethanolic extracts of seaweeds of C. toxifolia and C. peltata possesses lead compounds for development of antiplasmodial drugs.