Optical multibead arrays for simple and complex odor discrimination
Optical multibead arrays for simple and complex odor discrimination
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DOI：
10.1021/ac001137a
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发表时间：
2001-06-01
影响因子：
7.4
通讯作者：
Pearce, TC
中科院分区：
文献类型：
--
作者：
Albert, KJ;Walt, DR;Pearce, TC
基于光纤微珠的传感器阵列平台已被用于区分六种不同的气味和空气载气，在气味暴露之前、期间和之后监测了六种不同类型的微珠传感器，每种传感器的重复超过250次，以产生对每个传感器-分析物组合唯一的随时间变化的荧光响应模式。总共2683个传感器仅仅分析了它们的荧光变化，并对来自相同传感器珠的信号进行了平均，以提高信噪比。三种复合(咖啡豆)气味和三种纯(简单)气味(甲苯、丙酮、1，3-二硝基甲苯)在其最高相对浓度下的分析物分类率达到100%。当使用较低的气味浓度时，该系统对分析物的识别率高于85%。传感器对这些气味刺激的响应重复性也进行了统计量化，这对于定义整个系统的检测极限至关重要。这些结果首次证明了我们的除珠技术在不同稀释水平下区分不同气味类型的实用价值。
A fiber optic bead-based sensor array platform has been employed to discriminate between six different odors and air carrier gas, Six different bead sensor types, with over 250 replicates of each, were monitored before, during, and after odor exposure to produce time-dependent fluorescence response patterns that were unique for each sensor-analyte combination. A total of 2683 sensors mere analyzed with respect to changes in their fluorescence, and signals from identical sensor beads were averaged to improve signal-to-noise ratios. Analyte classification rates of 100% were achieved for three complex (coffee bean) odors and three pure (simple) odors (toluene, acetone, 1,3-dinitrotoluene) measured at their highest relative concentrations. When lower odor concentrations were employed, the system exhibited better than 85% classification rates for analyte discrimination. Sensor response repeatability to these odor stimuli has also been quantified statistically, which is vital in defining the detection limit of the overall system. These results demonstrate, for the first time, the utility of our bead away technology for discriminating between different odor types at various dilution levels.