Self-assembly of the light-harvesting complex of photosystem II (LHCII) on alkanethiol-modified gold electrodes
Self-assembly of the light-harvesting complex of photosystem II (LHCII) on alkanethiol-modified gold electrodes
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光系统 II (LHCII) 的光捕获复合物在烷硫醇修饰的金电极上的自组装
DOI：
10.1007/s11164-014-1822-3
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发表时间：
2014
影响因子：
3.3
通讯作者：
Mamoru Nango
中科院分区：
文献类型：
--
作者：
Masaharu Kondo;Mizuki Amano;Fujii Kaoru;,Ayumi Okuda;Shuichi Isigure;Takeshisa Dewa;Yutaka Amao;Hideki Hashimoto;Mamoru Nango
将从菠菜中分离的光系统II（LHCII）的捕光复合物固定到用烷硫醇、NH 2-（CH 2）n-SH、HOOC-（CH 2）7-SH和CH 3-（CH 2）7-SH的自组装单分子层（SAMs）修饰的金电极和裸电极上。NH_2-（CH_2）_n-SH表面处理对LHCII络合吸附的影响顺序为氨基>羧酸基>甲基，并随亚甲基链长的增加而增大。有趣的是，光电流密度依赖于NH_2-（CH_2）_n-SH中的端基和亚甲基链长，并按氨基>甲基>羧酸基团的顺序减小。在680 nm处照射时，观察到LHCII复合物对NH 2-（CH 2）n-SH（n= 8）的自组装膜的有效光电流响应。这些结果表明，LHCII配合物在氨基硫醇修饰的金电极的阳离子表面上被良好地组织。量子产率依赖于亚甲基链的长度（n），在n = 8时观察到最大光电流响应，这对应于NH_2-（CH_2）_8-SH中末端氨基与金表面之间的距离为1.7 nm。
A light-harvesting complex of photosystem II (LHCII), isolated from spinach, was immobilized onto a gold electrode modified with self-assembled monolayers (SAMs) of alkanethiols, NH2–(CH2)n–SH,n= 2, 6, 8, 11; HOOC–(CH2)7–SH; and CH3–(CH2)7–SH; and a bare electrode. The extent of LHCII complex adsorption according to surface treatment decreased in the order amino groups > carboxylic acid groups > methyl groups and increased with the methylene chain length in NH2–(CH2)n–SH. Interestingly, the photocurrent density depended on the terminal group and the methylene chain length in NH2–(CH2)n–SH and decreased in the order amino groups > methyl groups > carboxylic acid groups. An efficient photocurrent response of the LHCII complex on SAMs of NH2–(CH2)n–SH,n= 8 was observed upon illumination at 680 nm. These results indicated that the LHCII complexes were well organized on the cationic surfaces of the gold electrodes modified with amino alkanethiols. The quantum yield depended on the methylene chain length (n), where the maximum photocurrent response was observed atn= 8, which corresponded to a distance of 1.7 nm between the terminal amino group in NH2–(CH2)8–SH and the gold surface.