Critical roles for transcription factor GATA-3 in thymocyte development
Critical roles for transcription factor GATA-3 in thymocyte development
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DOI：
10.1016/s1074-7613(03)00328-5
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发表时间：
2003-12-01
期刊：
影响因子：
32.4
通讯作者：
Ho, IC
中科院分区：
文献类型：
--
作者：
Pai, SY;Truitt, ML;Ho, IC
转录因子加塔-3在胸腺发育的每个阶段都有表达，但其在胸腺细胞分化中的作用尚不清楚。缺乏加塔-3的RAG嵌合体动物不能从普通淋巴祖细胞产生早期胸腺细胞的事实迄今为止已经排除了对加塔-3在胸腺中的功能的研究。为了解决这个问题，我们在胸腺分化的早期和晚期产生了加塔-3缺陷的小鼠。我们的研究表明，加塔-3参与β选择，是不可或缺的单阳性CD 4胸腺细胞的发展。因此，我们的数据表明，协调和调节加塔-3在胸腺发育的每个阶段的表达是至关重要的成熟T细胞的产生。
The transcription factor GATA-3 is expressed at every stage of thymic development, but its role in thymocyte differentiation is unknown. The fact that RAG chimeric animals lacking GATA-3 cannot generate early thymocytes from common lymphoid progenitors has thus far precluded investigation of the function of GATA-3 in the thymus. To address this, we generated mice deficient in GATA-3 at early and late stages of thymic differentiation. Our studies revealed that GATA-3 is involved in beta selection and is indispensable for single-positive CD4 thymocyte development. Thus, our data demonstrate that the coordinated and regulated expression of GATA-3 at each stage of thymic development is critical for the generation of mature T cells.