Platelet-derived growth factor or basic fibroblast growth factor induce anchorage-independent growth of human fibroblasts.
Platelet-derived growth factor or basic fibroblast growth factor induce anchorage-independent growth of human fibroblasts.
复制标题
血小板源性生长因子或碱性成纤维细胞生长因子诱导人成纤维细胞的贴壁依赖性生长。
DOI：
10.1002/jcp.1041370328
复制
发表时间：
1988
影响因子：
5.6
通讯作者：
McCormick,JJ
中科院分区：
文献类型：
--
作者：
Palmer,H;Maher,VM;McCormick,JJ
锚定独立生长，即，半固体培养基中的生长被认为是成纤维细胞细胞转化的标志。二倍体人成纤维细胞通常不表现出这种生长，但当培养基含有高浓度的胎牛血清时可以短暂生长。这表明血清中的一些生长因子负责锚定非依赖性生长。已经做了大量工作来表征各种啮齿动物成纤维细胞对肽生长因子的需求，以实现非贴壁依赖性生长;然而，人成纤维细胞的需求尚不清楚。为了确定人成纤维细胞对于锚定非依赖性生长的肽生长因子需求，我们使用了含有已使其肽生长因子失活的血清的培养基。我们发现，血小板衍生生长因子（PDGF）或成纤维细胞生长因子（bFGF）的基本形式诱导锚定非依赖性生长。表皮生长因子（EGF）没有增强PDGF诱导的生长，或者只有轻微的增强。转化生长因子β（TGF-β）降低PDGF诱导的生长。EGF与TGF-β联合在半固体培养基中诱导集落形成，在此浓度下，生长因子本身都无效，但该组合在刺激非贴壁依赖性生长方面的效果远不如PDGF或bFGF。这项工作表明，PDGF或bFGF或EGF与TGF-β组合可以刺激非转化人成纤维细胞的锚定非依赖性生长。结果支持细胞转化可以减少或消除对外源性PDGF或bFGF的需要的想法。
Anchorage‐independent growth, i.e., growth in semi‐solid medium is considered a marker of cellular transformation of fibroblast cells. Diploid human fibroblasts ordinarily do not exhibit such growth but can grow transiently when medium contains high concentrations of fetal bovine serum. This suggests that some growth factor(s) in serum is responsible for anchorage‐independent growth. Much work has been done to characterize the peptide growth factor requirements of various rodent fibroblast cells for anchorage‐independent growth; however, the requirements of human fibroblasts are not known. To determine the peptide growth factor requirements of human fibroblasts for anchorage‐independent growth, we used medium containing serum that had had its peptide growth factors inactivated. We found that either platelet‐derived growth factor (PDGF) or the basic form of fibroblast growth factor (bFGF) induced anchorage‐independent growth. Epidermal growth factor (EGF) did not enhance the growth induced by PDGF, or did so only slightly. Transforming growth factor beta (TGF‐β) decreased the growth induced by PDGF. EGF combined with TGF‐β induced colony formation in semi‐solid medium at concentrations at which neither growth factor by itself was effective, but the combination was much less effective in stimulating anchorage‐independent growth than PDGF or bFGF. This work showed that PDGF, or bFGF, or EGF combined with TGF‐β can stimulate anchorage‐independent growth of nontransformed human fibroblasts. The results support the idea that cellular transformation may reduce or eliminate the need for exogenous PDGF or bFGF.