OCCUPATION OF THE EXTRACELLULAR-MATRIX RECEPTOR, INTEGRIN, IS A CONTROL POINT FOR MYOGENIC DIFFERENTIATION
OCCUPATION OF THE EXTRACELLULAR-MATRIX RECEPTOR, INTEGRIN, IS A CONTROL POINT FOR MYOGENIC DIFFERENTIATION
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DOI：
10.1016/0092-8674(87)90009-2
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发表时间：
1987-10-09
期刊：
影响因子：
64.5
通讯作者：
BOETTIGER, D
中科院分区：
文献类型：
--
作者：
MENKO, AS;BOETTIGER, D
用单克隆抗体CSAT处理可复制的鸡胚成肌细胞，该抗体可识别并阻断。积分素的亚基。在这种抗体存在的情况下，成肌细胞继续复制，不融合或产生肌肉特异性的肌凝蛋白。这种对正常分化的阻碍很容易通过去除抗体而逆转。逆转的细胞显示出促球蛋白和缩肌球蛋白的增加，并融合产生收缩肌管。这些数据表明，存在于复制性成肌细胞膜上的整合素与底物上的细胞外基质的相互作用对于启动成肌分化的最终阶段至关重要。
Replicating chicken embryo myoblasts were treated with a monoclonal antibody, CSAT, which recognizes and blocks the function of the .beta. subunit of integrin. In the presence of this antibody, myoblasts continued to replicate and did not fuse or produce muscle-specific meromyosin. This block to normal differentiation was readily reversed by the removal of the antibody. The reversed cells exhibited increases in desmin and meromyosin, and fused to produce contracting myotubes. The data indicate that the interaction of integrin, present on the membrane of replicating myoblasts, with extracellular matrix on the substrate is essential to initiate the terminal stages of myogenic differentiation.