Real microgravity condition promoted regeneration capacity of induced pluripotent stem cells during the TZ-1 space mission.
Real microgravity condition promoted regeneration capacity of induced pluripotent stem cells during the TZ-1 space mission.
复制标题
在TZ-1太空任务期间，真实的微重力条件促进了诱导多能干细胞的再生能力。
DOI：
10.1111/cpr.12574
复制
发表时间：
2019
影响因子：
8.5
通讯作者：
Wang Chang-Yong
中科院分区：
文献类型：
--
作者：
Zhou Jin;Dong Xiao-Hui;Zhang Feng-Zhi;Zhu Hui-Min;Hao Tong;Jiang Xiao-Xia;Zheng Wei-Bo;Zhang Tao;Wang Pei-Zhe;Li Hong;Na Jie;Wang Chang-Yong
诱导多能干细胞（iPSC）是经过重新编程的体细胞，具有与胚胎干细胞类似的自我更新和分化能力。借助天舟一号飞船任务的宝贵机会，我们研究了空间微重力（μg）对iPSC自我更新能力的影响。使用携带多能性报告基因 Oct4-GFP 的鼠 iPSC。将 Oct4-EGFP-iPSC 克隆装入生物反应器中，并在外太空暴露于 μg 环境中 14 天。对照实验是在相同的设备中进行的，但在地球重力（1 g）的地面上进行。 iPSC 克隆在推出前结构紧凑且高度表达 Oct4。在 μg 条件下，iPSC 克隆中的细胞在启动后的前 3 天内比在地面 1 g 条件下的细胞扩散得更快。然而，在 1 g 条件下，随着细胞密度的增加，Oct4-GFP 信号在接下来的 3 天内显着下降。有趣的是，在 μg 条件下，前 3 天内源自分散克隆的 iPSC 似乎聚集在一起并重新形成激活强 Oct4 表达的集落。另一方面，1 g 条件下的 iPSC 克隆在过度生长后无法恢复 Oct4 表达。我们的研究首次对太空中iPSC的增殖过程进行实时成像，发现在μg条件下，细胞行为似乎比地面上更加动态。
Induced pluripotent stem cells (iPSCs) are reprogrammed somatic cells that gained self‐renewal and differentiation capacity similar to embryonic stem cells. Taking the precious opportunity of the TianZhou‐1 spacecraft mission, we studied the effect of space microgravity (µg) on the self‐renewal capacity of iPSCs. Murine iPSCs carrying pluripotency reporter Oct4‐GFP were used. The Oct4‐EGFP‐iPSCs clones were loaded into the bioreactor and exposed to μg in outer space for 14 days. The control experiment was performed in identical device but on the ground in earth gravity (1 g). iPSCs clones were compact and highly expressed Oct4 before launch. In μg condition, cells in iPSC clones spread out more rapidly than those in ground 1 g condition during the first 3 days after launch. However, in 1 g condition, as the cell density increases, the Oct4‐GFP signal dropped significantly during the following 3 days. Interestingly, in μg condition, iPSCs originated from the spread‐out clones during the first 3 days appeared to cluster together and reform colonies that activated strong Oct4 expression. On the other hand, iPSC clones in 1 g condition were not able to recover Oct4 expression after overgrown. Our study for the first time performed real‐time imaging on the proliferation process of iPSCs in space and found that in μg condition, cell behaviour appeared to be more dynamic than on the ground.