Combined molecular and morphological phylogeny of Ephemerellinae (Ephemerellidae: Ephemeroptera), with remarks about classification
Combined molecular and morphological phylogeny of Ephemerellinae (Ephemerellidae: Ephemeroptera), with remarks about classification
复制标题
DOI：
10.11646/zootaxa.1991.1.2
复制
发表时间：
2009-01-30
期刊：
影响因子：
0.9
通讯作者：
Whiting, Michael F.
中科院分区：
文献类型：
--
作者：
Ogden, T. Heath;Osborne, Jonathan T.;Whiting, Michael F.
本研究首次将分子与形态相结合的分析方法应用于叶蝉亚科叶蝉亚科的分类研究。根据3个核基因(18S rDNA、28S rDNA、组蛋白H3)和2个线粒体基因(12S rDNA、16S rDNA)的DNA序列数据和23个形态特征构建了系统发育树。分类单元样本包括现存的所有25个属组的样本和具有最高多样性的属的其他代表。眼镜蛇科似乎由三个主要支系组成。Ephmerella，Ephmerellidae的最大属，和Serratella没有被支持为单系，每一个都在三个主要支系中的两个中有代表。而Drunella属和Cincticoella属被支持为单系分类。被支持为单系的谱系包括Timpanoga属、Dannella属、Dentatella属和Eurylopella属，以及Ephmerellinae属Torleya、Hyrtanella和Crinitella和Kangella属、Uracanthella属和Teloganopsis属。根据分子和组合数据，该属被归入麻黄藻亚科，但仅根据形态数据将其与其他亚科归类。如果要反映系统发育关系，还需要进一步研究和分析斑点藻科的形态，并修改分类。
This study represents the first combined molecular and morphological analysis for the mayfly family Ephemerellidae (Ephemeroptera), with a focus on the relationships of genera and species groups of the subfamily Ephemerellinae. The phylogeny was constructed based on DNA sequence data from 3 nuclear (18S rDNA, 28S rDNA, histone H3) and 2 mitochondrial (12S rDNA, 16S rDNA) genes, and 23 morphological characters. Taxon sampling for Ephemerellidae included exemplars from all 25 extant genus groups and additional representatives from those genera with the highest diversity. Ephemerellidae appears to consist of three major clades. Ephemerella, the largest genus of Ephemerellidae, and Serratella were not supported as monophyletic, and each had representatives in two of the three major clades. However, the genera Drunella and Cincticostella were supported as monophyletic. Lineages strongly supported as monophyletic include a grouping of the Timpanoginae genera Timpanoga, Dannella, Dentatella and Eurylophella, and groupings of the Ephemerellinae genera Torleya, Hyrtanella and Crinitella and the genera Kangella, Uracanthella and Teloganopsis. The placement of the Timpanoginae genus Attenella fell within Ephemerellinae, based on molecular and combined data, but it grouped with other Timpanoginae based on morphological data alone. Further study and analysis of Ephemerellidae morphology is needed, and classification should be revised, if it is to reflect phylogenetic relationships.