Virion structure.
Virion structure.
复制标题
病毒体结构。
DOI：
10.1007/978-3-642-76018-1_3
复制
发表时间：
1991
影响因子：
--
通讯作者：
Chiu,W
中科院分区：
文献类型：
--
作者：
Hewlett,MJ;Chiu,W
A detailed three-dimensional image of a virus particle is essential for understanding its mechanism of assembly, its antigenic properties, its interaction with receptors (RossmannandReuckert1987;Harrison1990) and for the formation of a biophysical basis for rational design of antiviral agents (Rossmann1989). X-ray crystallographic analysis has resulted in the structural solution, at the atomic level, of several nonenveloped animal viruses (Hogleet al. 1985;Rossmannet al. 1985;Achryaet al. 1989). The diameters of these viruses are smaller than 400 Å. So far, its has been difficult to grow crystals of larger viruses with sufficient diffraction quality. Electron cryomicroscopy coupled with image analysis has emerged as a useful technique for providing three-dimensional structures of icosahedral virus particles which cannot readily form large crystalline arrays (Adrianet al. 1984;Vogelet al. 1986;Prasadet al. 1988;Bakeret al. 1988;Schraget al. 1989). The availability of monospecific antisera directed against viral capsid proteins has permitted the use of these reagents to locate the domains of antigenic sites on the surface of viral capsid proteins (Prasadet al. 1990).
