Patterns of sheath elongation, cell proliferation, and manganese(II) oxidation in Leptothrix cholodnii
Patterns of sheath elongation, cell proliferation, and manganese(II) oxidation in Leptothrix cholodnii
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DOI：
10.1007/s00203-012-0801-6
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发表时间：
2012-03
影响因子：
2.8
通讯作者：
M. Takeda;Yuta Kawasaki;Takuto Umezu;S. Shimura;M. Hasegawa;J. Koizumi
中科院分区：
文献类型：
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作者：
M. Takeda;Yuta Kawasaki;Takuto Umezu;S. Shimura;M. Hasegawa;J. Koizumi
Leptothrix cholodniii 是 β-变形菌纲的 Mn(II) 氧化和鞘形成成员。它的鞘是包含细胞链的微管状丝。从化学角度来看，鞘可以被描述为由富含半胱氨酸的聚合糖复合物（称为硫肽聚糖）组成的超分子。然而，控制鞘长度增加的机制尚不清楚。在本研究中，我们尝试使用常规试剂通过显微镜检查来检测护套伸长率。使用生物素缀合的马来酰亚胺、丙酸缀合的马来酰亚胺和荧光抗生物素抗体的组合来完成对鞘的预先存在的或新形成的区域的选择性荧光标记。落射荧光显微镜表明鞘在末端区域伸长。基于这一观察，我们假设新分泌的硫肽聚糖分子在其末端整合到预先存在的鞘中。连续的相差显微镜显示，所有细胞都以几乎相同的速度增殖，无论它们在鞘内的位置如何。还根据培养时间检查了微培养物中的 Mn(II) 氧化。结果表明，Mn氧化物的沉积在老化区域显着。组合数据揭示了 L 中鞘伸长、细胞增殖和锰氧化物沉积之间的时空关系。科洛德尼。
Leptothrix cholodniiis a Mn(II)-oxidizing and sheath-forming member of the class β-Proteobacteria.Its sheath is a microtube-like filament that contains a chain of cells. From a chemical perspective, the sheath can be described as a supermolecule composed of a cysteine-rich polymeric glycoconjugate, called thiopeptidoglycan. However, the mechanism that controls the increase in sheath length is unknown. In this study, we attempted to detect sheath elongation through microscopic examination by using conventional reagents. Selective fluorescent labeling of preexisting or newly formed regions of the sheath was accomplished using combinations of biotin-conjugated maleimide, propionate-conjugated maleimide, and a fluorescent antibiotin antibody. Epifluorescence microscopy indicated that the sheath elongates at the terminal regions. On the bases of this observation, we assumed that the newly secreted thiopeptidoglycan molecules are integrated into the preexisting sheath at its terminal ends. Successive phase-contrast microscopy revealed that all cells proliferate at nearly the same rate regardless of their positions within the sheath. Mn(II) oxidation in microcultures was also examined with respect to cultivation time. Results suggested that the deposition of Mn oxides is notable in the aged regions. The combined data reveal the spatiotemporal relationships among sheath elongation, cell proliferation, and Mn oxide deposition inL. cholodnii.