Subfunctionalization of the Ruby2-Ruby1 gene cluster during the domestication of citrus
Subfunctionalization of the Ruby2-Ruby1 gene cluster during the domestication of citrus
复制标题
柑橘驯化过程中 Ruby2–Ruby1 基因簇的亚功能化
DOI：
10.1038/s41477-018-0287-6
复制
发表时间：
2018-11-01
期刊：
影响因子：
18
通讯作者：
Xu, Qiang
中科院分区：
文献类型：
--
作者：
Huang, Ding;Wang, Xia;Xu, Qiang
植物果实颜色的进化很有趣。柑橘类水果反复获得或失去合成花青素的能力。橙子是一种原始的柑橘属植物，其果实和叶片都能积累花青素。野生柑橘可以在其叶片中积累花青素。相比之下，大多数栽培柑橘已经失去了积累花青素的能力。我们的特点是一个新的MYB调控基因，Ruby 2，这是相邻的Ruby 1，一个已知的柑橘花青素激活剂。不同的Ruby 2等位基因对花青素苷生物合成的调控可能具有相反的作用。AbRuby 2Full编码一种主要在中国箱橙子色素叶中起作用的花色素苷激活剂。CgRuby 2Short在紫柚中被鉴定出，编码一个花色苷阻遏蛋白。CgRuby 2Shorthas失去了激活花青素苷生物合成的能力。然而，它保留了与CgRuby 1相同的合作伙伴CgbHLH 1互动的能力，因此在监管复合体中充当被动竞争对手。在不同柑橘物种中的进一步研究表明，Ruby 2-Ruby 1簇在原始，野生和栽培柑橘中表现出亚功能化。我们的研究阐明了柑橘中Ruby 2-Ruby 1簇的调控机制和进化历史，这是独特的，不同于拟南芥，葡萄或矮牵牛。
The evolution of fruit colour in plants is intriguing. Citrus fruit has repeatedly gained or lost the ability to synthesize anthocyanins. Chinese box orange, a primitive citrus, can accumulate anthocyanins both in its fruits and its leaves. Wild citrus can accumulate anthocyanins in its leaves. In contrast, most cultivated citrus have lost the ability to accumulate anthocyanins. We characterized a novel MYB regulatory gene, Ruby2, which is adjacent to Ruby1, a known anthocyanin activator of citrus. Different Ruby2 alleles can have opposite effects on the regulation of anthocyanin biosynthesis. AbRuby2Fullencodes an anthocyanin activator that mainly functions in the pigmented leaves of Chinese box orange. CgRuby2Shortwas identified in purple pummelo and encodes an anthocyanin repressor. CgRuby2Shorthas lost the ability to activate anthocyanin biosynthesis. However, it retains the ability to interact with the same partner, CgbHLH1, as CgRuby1, thus acting as a passive competitor in the regulatory complex. Further investigation in different citrus species indicated that the Ruby2-Ruby1 cluster exhibits subfunctionalization among primitive, wild and cultivated citrus. Our study elucidates the regulatory mechanism and evolutionary history of the Ruby2-Ruby1 cluster in citrus, which are unique and different from that found in Arabidopsis, grape or petunia.