Effect of respiratory muscle training on GLUT-4 in the sheep diaphragm.
Effect of respiratory muscle training on GLUT-4 in the sheep diaphragm.
复制标题
呼吸肌训练对绵羊膈肌GLUT-4的影响。
DOI：
10.1097/00005768-200008000-00008
复制
发表时间：
2000
影响因子：
4.1
通讯作者：
Bazzy,AR
中科院分区：
文献类型：
--
作者：
Bhandari,A;Xia,Y;Cortright,R;Dohm,GL;Bazzy,AR
PurposeEndurance exercise training is associated with enhanced glucose uptake and hence improvement in carbohydrate metabolism. Glucose transport (GLUT) membrane proteins are regulated by a variety of physiological stimuli, including exercise. In limb muscle, both acute exercise and endurance training enhance the expression of the skeletal muscle transporter, GLUT-4. The purpose of this study is to determine whether chronic loading enhances GLUT-4 expression in the diaphragm.MethodsThe effect of chronic inspiratory flow resistive (IFR) loading on diaphragm GLUT-4 was studied in a model of respiratory muscle endurance training in sheep. IFR loads (resistance 50-100 cm H2O x L (-1) xs (-1)) were maintained for 3 hxd (-1), 5-6 dx wk (-1) for 3 wk. Loading was adjusted so that PaO2 was> 60 Torr and PaCO2< 45 Torr in room air. Six untrained sheep were used as controls. GLUT-4 protein and mRNA were analyzed by Western and Northern analysis respectively.ResultsGLUT-4 protein levels were two-fold greater in trained animals when compared with controls (P< 0.01). GLUT-4 mRNA levels in the trained muscles was not significantly different from controls.ConclusionsWe conclude that in the sheep diaphragm, chronic IFR loading increases GLUT-4 protein levels. This increase may be one of the mechanisms contributing to the improved respiratory muscle endurance previously demonstrated in this animal model of respiratory muscle training.
