Identification of WHIRLY1 as a Factor Binding to the Promoter of the Stress- and Senescence-Associated Gene HvS40
Identification of WHIRLY1 as a Factor Binding to the Promoter of the Stress- and Senescence-Associated Gene HvS40
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DOI：
10.1007/s00344-013-9378-9
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发表时间：
2014-03-01
影响因子：
4.8
通讯作者：
Buck, Friedrich
中科院分区：
文献类型：
--
作者：
Krupinska, Karin;Daehnhardt, Dorothee;Buck, Friedrich
HvS40是大麦叶片衰老的标记基因。它在黑暗诱导的衰老和年龄依赖性衰老以及对生物和非生物胁迫因素的反应中表达。它的表达也由参与控制应激反应和衰老过程的激素诱导。对分离细胞核的连续转录测定表明，其在黑暗诱导衰老期间的表达在转录水平上受到调节。它的启动子含有两个W盒组成的激发子应答元件（ERE）。蛋白质与这些基序的结合在衰老过程中发生了变化。一个24 kDa的蛋白结合到非衰老叶片的ERE 2被确定为WHIRLY1。
HvS40 is a marker gene for leaf senescence in barley. It is expressed during dark-induced as well as age-dependent senescence and in response to biotic and abiotic stress factors. Its expression is also induced by hormones involved in the control of stress responses and senescence processes. Run-on-transcription assays with isolated nuclei showed that its expression during dark-induced senescence is regulated at the transcriptional level. Its promoter contains two elicitor response elements (ERE) consisting of two W-boxes. Binding of proteins to these motifs changed during senescence. A 24-kDa protein binding to ERE2 of nonsenescent leaves was identified as WHIRLY1.