A complementary density gradient of zwitterionic polymer brushes and NCAM peptides for selectively controlling directional migration of Schwann cells
A complementary density gradient of zwitterionic polymer brushes and NCAM peptides for selectively controlling directional migration of Schwann cells
复制标题
两性离子聚合物刷和 NCAM 肽的互补密度梯度用于选择性控制雪旺细胞的定向迁移
DOI：
10.1016/j.biomaterials.2015.03.052
复制
发表时间：
2015-07-01
期刊：
影响因子：
14
通讯作者：
Gao, Changyou
中科院分区：
文献类型：
--
作者：
Ren, Tanchen;Yu, Shan;Gao, Changyou
选择性增强雪旺细胞(SCs)在成纤维细胞(FIB)上的定向迁移在周围神经再生中起着重要作用，因为这种行为有助于神经元修复和避免纤维化。本文制备了聚(3-二甲基甲基丙烯酰氧乙基丙烷磺酸铵)(PDMAPS，一种具有防污性能的两性聚合物)和KHIFSDDSSE多肽(KHIFSDDSSE多肽，源于介导细胞黏附的神经细胞黏附分子NCAM)的互补密度梯度。用荧光标记法显示梯度，并用X射线光电子能谱(XPS)和耗散石英晶体微天平(QCM-d)进一步表征。SCs在梯度表面向PDMAPS密度较低和KHI多肽密度较高的区域有择优取向和增强的定向迁移，而FIB则表现为随机迁移。此外，SCs在玻璃上的迁移速率比在玻璃上自然状态下的迁移速率显著提高2倍，而FIBS的迁移速率降低到60%，导致SCs的迁移速度比FIBS更快。互补梯度的成功依赖于PDMAPS刷子和细胞特异性配体之间的适当相互作用，从而能够选择性地指导干细胞的迁移。(C)2015爱思唯尔有限公司。保留所有权利。
Selective enhancement of directional migration of Schwann cells (SCs) over fibroblasts (FIBS) plays a significant role in peripheral nerve regeneration, because this behavior facilitates neuron repair and avoids fibrosis. Herein a complementary density gradient of poly(3-dimethyl-methacryloyloxyethyl ammonium propane sulfonate) (PDMAPS, a zwitterionic polymer with antifouling property) and KHIFSDDSSE peptide (KHI, derived from neural cell adhesion molecule NCAM which mediates cell cell adhesion) was fabricated. The gradient was visualized by fluorescent labeling, and further characterized by X-ray photoelectron spectrometry (XPS) and quartz crystal microbalance with dissipation (QCM-d). The SCs exhibited preferential orientation and enhanced directional migration on the gradient surface toward the region of lower PDMAPS density and higher KHI peptide density, while FIBs showed random migration. Moreover, the migration rate of the SCs was significantly enhanced to 2 folds, whereas that of the FIBs was reduced to 60% compared to their natural state on glass, leading to a faster migration rate of SCs than FIBs. The success of the complementary gradient relies on the appropriate interplay between the PDMAPS brushes and the cell-specific ligands, enabling the selective guidance of SCs migration. (C) 2015 Elsevier Ltd. All rights reserved.