Differential expression of interleukin-8 and intercellular adhesion molecule-1 by human gingival epithelial cells in response to Actinobacillus actinomycetemcomitans or Porphyromonas gingivalis infection
Differential expression of interleukin-8 and intercellular adhesion molecule-1 by human gingival epithelial cells in response to Actinobacillus actinomycetemcomitans or Porphyromonas gingivalis infection
复制标题
人牙龈上皮细胞在伴放线聚集杆菌或牙龈卟啉单胞菌感染刺激下白细胞介素 - 8和细胞间黏附分子 - 1的差异表达
DOI：
10.1111/j.1399-302x.1998.tb00711.x
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发表时间：
1998-10-01
影响因子：
--
通讯作者：
Park, NH
中科院分区：
文献类型：
--
作者：
Huang, GTJ;Haake, SK;Park, NH
牙龈上皮细胞表达的分子在与牙周病原体相互作用后参与启动和维持炎症反应，目前知之甚少。因此，我们研究了伴生放线杆菌和牙龈卟啉单胞菌感染对牙周上皮细胞中性粒细胞趋化因子IL-8和黏附分子细胞间黏附分子-1表达的影响。结果显示，伴随放线菌感染后，IL-8和细胞间黏附分子-1的表达均增加(IL-8：2~7倍；细胞间黏附分子-1：2.5~3.7倍)。IL-8的分泌在感染后6h达到高峰，随后表达降至基础水平。细胞表面细胞间黏附分子-1的表达在感染后4h开始升高，14h达到高峰。反之，两种分子的表达在感染牙龈假单胞菌后2小时迅速下降。伴生放线杆菌和牙龈假单胞菌感染对牙龈上皮细胞IL-8和细胞间黏附分子-1表达的相反影响表明，伴生放线杆菌感染可能启动中性粒细胞的募集，而牙龈放线杆菌感染可能延缓这一过程，从而显示出独特的毒力前景。
Little is known regarding the molecules expressed by gingival epithelial cells that are involved in initiating and maintaining inflammation following the interaction with periodontal pathogens. Thus, we investigated the effect pf Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis infection on the expression of neutrophil chemoattractant interleukin 8 (IL-8) and the adhesion molecule intercellular adhesion molecule-1 by gingival epithelial cells. The data revealed that both IL-8 and intercellular adhesion molecule-1 expression increased after infection with A. actinomycetemcomitans (IL-8: 2- to 7-fold; intercellular adhesion molecule-1: 2.5- to 3.7-fold). IL-8 secretion reached a maximal level 6 h after the infection and the expression subsequently decreased to basal level. The increased cell surface intercellular adhesion molecule-1 expression started at 4 h after infection and reached a maximal level 14 h after the infection. In contrast, the expression of both molecules rapidly decreased 2 h after challenge with P. gingivalis. This opposite influence of A. actinomycetemcomitans and P. gingivalis infection on the expression of IL-8 and intercellular adhesion molecule-1 by gingival epithelial cells suggests that A. actinomycetemcomitans infection may initiate the recruitment of neutrophils, whereas the P. gingivalis infection may retard this process and therefore demonstrate a distinct perspective of virulence.