Interfacing biology with nanoparticles
Interfacing biology with nanoparticles
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DOI：
10.1016/j.cap.2004.06.006
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发表时间：
2005-02-01
影响因子：
2.4
通讯作者：
Sastry, M
中科院分区：
文献类型：
--
作者：
Mandal, S;Phadtare, S;Sastry, M
无机纳米材料的合成及其表面改性在传感和电子应用方面的新方法不断发展。在金纳米粒子的表面修饰方面，巯基化学是最常用的将配体结合到其表面的方法。我们一直在寻求使用胺官能团将配体结合到金纳米颗粒表面的可能性，并且已经发现胺结合与硫醇结合一样强。使用胺化学对纳米金进行表面改性的优点有很多，其中最重要的一个是可以络合多种生物分子，如氨基酸和蛋白质。在这篇文章中，我们回顾了本实验室的工作，使用氨基酸以及使用氨基酸作为还原剂，以获得稳定的水溶液的可变大小的金纳米粒子的稳定的金纳米粒子。我们还讨论了将金纳米粒子修饰在聚合物微球表面形成酶的生物偶联物的可能性及其作为可重复使用的生物催化剂的应用。通过将纳米材料与生物学结合，可以获得很多好处，在纳米技术和生物技术方面都可能产生相当大的副产品。(C)2004 Elsevier B.V.保留所有权利。
New approaches are constantly being developed for both the synthesis of inorganic nanomaterials and their surface modification for sensing and electronic applications. Insofar as surface modification of gold nanoparticles is concerned, thiol chemistry is the most popular approach to bind ligands to their surface. We have been pursuing the possibility of using amine functionality to bind ligands to the surface of gold nanoparticles and have found that amine binding is as strong as thiol binding. The advantages of using amine chemistry for surface modification of nanogold are many, the possibility of complexing a large variety of biomolecules such as amino acids and proteins being one of the most important. In this article, we review the work from this laboratory on the stabilization of gold nanoparticles using amino acids as well as using amino acids as reducing agents to obtain stable aqueous solutions of gold nanoparticles of variable size. We also discuss the possibility of forming bioconjugates of enzymes with gold nanoparticles decorating the surface of polymer microspheres and their application as reusable biocatalysts. There is much to be gained by marrying nanomaterials with biology with considerable spin-offs likely in both nanotechnology and biotechnology. (C) 2004 Elsevier B.V. All rights reserved.