Comparison of cytokines in the peritoneal fluid and conditioned medium of adolescents and adults with and without endometriosis
Comparison of cytokines in the peritoneal fluid and conditioned medium of adolescents and adults with and without endometriosis
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DOI：
10.1111/aji.13347
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发表时间：
2020-09-29
影响因子：
3.6
通讯作者：
Missmer, Stacey A.
中科院分区：
文献类型：
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作者：
Bailey, Amelia P.;Hill, Abby S.;Missmer, Stacey A.
问题比较有和没有子宫内膜异位症的青少年和成人的炎症和免疫相关的腹膜细胞因子。研究方法在多个大学附属科学中心进行的巢式病例对照研究中，10名青少年和13名经视觉和组织学证实的子宫内膜异位症成人（例），13名经视觉怀疑子宫内膜异位症但不确定的青少年（例），（7例患者）或阴性（6名患者）组织学，和15名因非恶性妇科疾病而接受手术的成年人，（对照组）进行腹腔镜抽吸腹膜液（PF），从中测定PF和条件培养基（CM）细胞因子水平。结果与成人子宫内膜异位症患者相比，MCP-3、IL-12 p40、MIP-1 β和IL-15在青少年子宫内膜异位症患者中显著升高，而TNF-β和CTACK在青少年子宫内膜异位症患者中显著降低。除了MIP-1 β无统计学差异外，这些差异与青少年子宫内膜异位症患者和成年对照组相似。然而，MIP-1 β是观察到的在成人病例和对照组之间存在差异的唯一细胞因子。三组间CM细胞因子无显著差异。当分析仅限于（a）月经周期第1-10天，（B）未暴露于外源性激素的患者，或（c）无论是否存在组织学子宫内膜异位症的所有青少年时，结果相似。结论：观察到6种PF细胞因子的生物学相关性和统计学显著性差异，提示青少年子宫内膜异位症患者中更有利于侵袭的细胞因子谱。与有或无子宫内膜异位症的成人相比，患有子宫内膜异位症的青少年具有独特的腹膜细胞因子谱和分子行为。
Problem To compare inflammatory- and immune-associated peritoneal cytokines of adolescents and adults with and without endometriosis. Methods of study In a nested case-control study in multiple university-affiliated scientific centers, ten adolescents and thirteen adults with visually and histologically confirmed endometriosis (cases), thirteen adolescents with visually suspected endometriosis but indeterminate (seven patients) or negative (six patients) histology, and fifteen adults undergoing surgery for non-malignant gynecologic disease without endometriosis (controls) underwent laparoscopic aspiration of peritoneal fluid (PF), from which PF and conditioned medium (CM) cytokine levels were assayed. Results Compared to adults with endometriosis, MCP-3, IL-12p40, MIP-1 beta, and IL-15 were significantly higher among adolescents with endometriosis, while TNF-beta and CTACK were lower among adolescents. These differences were similar comparing adolescents with endometriosis to adult controls except for MIP-1 beta, which was not statistically different. MIP-1 beta was, however, the only cytokine observed to differ between adult cases and controls. There were no significant differences in CM cytokines among the three groups. Results were similar when analyses were restricted to samples collected (a) during menstrual cycle days 1-10, (b) from patients unexposed to exogenous hormones, or (c) from all adolescents despite presence or absence of histologic endometriosis. Conclusion Biologically relevant and statistically significant differences in six PF cytokines were observed and suggest a more pro-invasion cytokine profile among adolescents with endometriosis. Adolescents with endometriosis have unique peritoneal cytokine profiles and molecular behavior when compared to adults with and without endometriosis.