Novel effects of diosgenin on skin aging
Novel effects of diosgenin on skin aging
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DOI：
10.1016/j.steroids.2009.01.006
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发表时间：
2009-06-01
期刊：
影响因子：
2.7
通讯作者：
Watanabe, Shinichi
中科院分区：
文献类型：
--
作者：
Tada, Yayoi;Kanda, Naoko;Watanabe, Shinichi
薯蓣或长毛薯蓣的提取物被用作更年期时的补充保健食品。这些提取物含有大量的植物类固醇，薯蓣皂苷元。在这里，我们研究了薯蓣皂苷元对抗更年期皮肤老化的安全性和功效。在体外，薯蓣皂苷元增强了人体 3D 皮肤等效模型中的 DNA 合成，并增加了成人角质形成细胞中溴脱氧尿苷的摄取和细胞内 cAMP 水平。薯蓣皂苷元对溴脱氧尿苷摄取的增加被腺苷酸环化酶抑制剂阻断，但不能被针对雌激素受体α、雌激素受体β或孤儿G蛋白偶联受体GPR30的反义寡核苷酸阻断，表明cAMP参与，但雌激素受体α、雌激素受体β或GPR30不参与。在体内，给予薯蓣皂苷元可以改善更年期模型卵巢切除小鼠的表皮厚度，但不会改变脂肪积累的程度。为了检查薯蓣皂苷元的安全性，将薯蓣皂苷元和17β-雌二醇给予患有乳腺癌的小鼠。结果显示，虽然17β-雌二醇加速了肿瘤生长，但薯蓣皂苷元却没有表现出这种作用。我们的发现，老化皮肤角质形成细胞增殖的恢复，表明薯蓣皂苷元可能具有作为更年期安全保健食品的潜力。 (C) 2009 Elsevier Inc. 保留所有权利。
Extracts of Dioscorea coomposita or Dioscorea villosa are consumed as supplemental health foods at the time of climacteric. The extracts contain large amounts of the plant steroid, diosgenin. Here, we studied the safety and efficacy of diosgenin against skin aging at the time of climacteric. In vitro, diosgenin enhanced DNA synthesis in a human 3D skin equivalent model, and increased bromodeoxyuridine uptake and intracellular cAMP level in adult human keratinocytes. The increase of bromodeoxyuridine uptake by diosgenin was blocked by an adenylate cyclase inhibitor, but not by antisense oligonucleotides against estrogen receptor alpha, estrogen receptor beta or an orphan G-protein-coupled receptor, GPR30, indicating the involvement of cAMP but not estrogen receptor alpha, estrogen receptor beta or GPR30. In vivo, administration of diosgenin improved the epidermal thickness in the ovariectomized mice, a climacteric model, without altering the degree of fat accumulation. in order to examine the safety of diosgenin, diosgenin and 17 beta-estradiol were administered to breast cancer-burdened mice. The results revealed that while 17 beta-estradiol accelerated the tumor growth, diosgenin did not show this effect. Our finding, a restoration of keratinocyte proliferation in aged skin, suggests that diosgenin may have potential as a safe health food for climacteric. (C) 2009 Elsevier Inc. All rights reserved.