Frequent infection of wild boar with atypical porcine pestivirus (APPV)
Frequent infection of wild boar with atypical porcine pestivirus (APPV)
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DOI：
10.1111/tbed.12854
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发表时间：
2018-08-01
影响因子：
4.3
通讯作者：
Postel, A.
中科院分区：
文献类型：
--
作者：
Cagatay, G. N.;Antos, A.;Postel, A.
新近发现的非典型猪瘟病毒（APPV）是引起新生仔猪先天性震颤的主要病原。尽管其相关性和广泛分布在家猪中，但迄今为止对野猪的情况一无所知，野猪是相关经典猪瘟病毒的重要野生动物宿主。在这项研究中，从北方德国获得的456野猪血清样品进行了调查的存在下，APPV基因组和病毒特异性抗体。实时RT-PCR分析结果显示基因组检测率为19%。来自不同狩猎区的APPV（n=12）的遗传特征表明，除了来自一个地点的APPV外，它们之间的遗传关系密切，在分析的部分NS 3编码区中的差异小于3.3%。此外，间接E-rns ELISA显示抗体检出率约为100%。52%，与病毒血症野猪的高数量一致。用E-rns抗体ELISA对来自塞尔维亚共和国的15个野猪样本进行分析，证明APPV在德国以外的野猪种群中也很丰富。大量的基因组和血清学阳性动物表明，野猪可能作为一个重要的病毒水库APPV。
The recently identified atypical porcine pestivirus (APPV) was demonstrated to be the causative agent of the neurological disorder congenital tremor in newborn piglets. Despite its relevance and wide distribution in domestic pigs, so far nothing is known about the situation in wild boar, representing an important wild animal reservoir for the related classical swine fever virus. In this study, 456 wild boar serum samples obtained from northern Germany were investigated for the presence of APPV genomes and virus-specific antibodies. Results of real-time RT-PCR analyses revealed a genome detection rate of 19%. Subsequent genetic characterization of APPV (n=12) from different hunting areas demonstrated close genetic relationship and, with exception of APPV from one location, displayed less than 3.3% differences in the analysed partial NS3 encoding region. Furthermore, indirect E-rns ELISA revealed an antibody detection rate of approx. 52%, being in line with the high number of viremic wild boar. Analysis of fifteen wild boar samples from the Republic of Serbia by E-rns antibody ELISA provided evidence that APPV is also abundant in wild boar populations outside Germany. High number of genome and seropositive animals suggest that wild boar may serve as an important virus reservoir for APPV.