Prevalence and genetic diversity of noroviruses in outpatient pediatric clinics in Beijing, China 2010-2012
Prevalence and genetic diversity of noroviruses in outpatient pediatric clinics in Beijing, China 2010-2012
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DOI：
10.1016/j.meegid.2014.09.006
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发表时间：
2014-12-01
影响因子：
3.2
通讯作者：
Dong, Hui-jin
中科院分区：
文献类型：
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作者：
Jia, Li-ping;Qian, Yuan;Dong, Hui-jin
诺沃克病毒是腹泻疾病的主要原因，在全世界所有年龄段的人类中都有流行、暴发或零星的模式。本研究旨在了解北京地区婴幼儿诺如病毒的基因分型特征。粪便标本(n=1128)取自2010-2012年3年间有急性胃肠炎症状的患者。RT-PCR检测诺如病毒阳性率为16.1%(182/1128)，其中聚合酶区122例，衣壳区177例，聚合酶和衣壳两区同时阳性117例。通过衣壳基因的序列分析，所有鉴定的诺如病毒都属于基因组II(GII)。在这些阳性样本中，以GII.4型(61.0%)最常见，其次是GII.3型(35.0%)。新的变种GII.4悉尼_2012株于9月份在本研究中出现，并在后来成为优势基因。对182份RT-PCR阳性标本中的117份标本进行了聚合酶基因和衣壳基因的基因分型。有趣的是，在这117例阳性标本中，有59例(50.4%)存在聚合酶和衣壳基因的错配，其中包括7种可疑的重组子模式。其中，GII.P12/GII.3是最常见的组合，占54.2%(32/59)，其次是GII.Pe/GII.4悉尼-2012，占23.7%(14/59)。在本研究中首次检测到两个新的重组体：GII.P22/GII.5和GII.21/GII.3。综上所述，本研究基于实验室数据，对北京地区急性胃肠炎患儿中诺如病毒的遗传多样性进行了详细描述。此外，数据显示，在诺沃克病毒的进化过程中，新的变种和新的重组经常出现。(C)2014爱思唯尔B.V.保留所有权利。
Norovirus is a major cause of diarrheal disease with epidemic, outbreak or sporadic patterns in humans of all ages worldwide. This study aimed to determine the genotypic characteristics of noroviruses from infants and children in Beijing. Stool samples (n = 1128) were collected from patients with symptoms of acute gastroenteritis in the past 3 years from 2010 to 2012. The norovirus positivity rate was 16.1% (182/1128) by using RT-PCR, including 122 with primer set covering polymerase region, 177 with primer set covering capsid region, and 117 with both polymerase and capsid regions. By sequence analysis for capsid genes, all the noroviruses identified were belonging to genogroup II (GII). Among these positive samples, GII.4 (61.0%) was the most common genotype detected, followed by GII.3 (35.0%). The new variant GII.4 Sydney_2012 strains emerged in this study in September and became the predominant genotype later. Those 117 from 182 RT-PCR positive samplers were able to be genotyped based on the sequences of both polymerase and capsid genes. The result was interesting that 59 out of these 117 positive specimens (50.4%) had mismatched genotypes between polymerase and capsid genes, including 7 suspected recombinants patterns. Among them, GII.P12/GII.3 was the most common combination which accounts for 54.2% (32/59), followed by GII.Pe/GII.4 Sydney_2012 which was 23.7% (14/59). Two novel recombinants, GII.P22/GII.5 and GII.21/GII.3 were first detected in this study. In summary, this study provides a detailed description based on laboratory data of the genetic diversity of norovirus in young children with acute gastroenteritis in Beijing. Moreover the data revealed that in the evolution of norovirus, new variant and novel recombination emerged frequently. (C) 2014 Elsevier B.V. All rights reserved.