SURFACE-PLASMON RESONANCE IMMUNOSENSORS - SENSITIVITY CONSIDERATIONS
SURFACE-PLASMON RESONANCE IMMUNOSENSORS - SENSITIVITY CONSIDERATIONS
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DOI：
10.1016/s0003-2670(00)81337-9
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发表时间：
1988-10-15
影响因子：
6.2
通讯作者：
GREVE, J
中科院分区：
文献类型：
--
作者：
KOOYMAN, RPH;KOLKMAN, H;GREVE, J
考虑了在免疫传感应用中提高表面等离子体共振响应的一些方面。从计算和实验中发现，对于与待定量物质直接接触的约55 nm厚的银层，获得最大灵敏度。具有高介电常数的中间层的应用可用于抑制背景响应。实验上，蛋白质表面覆盖率约为。0.1可以测量，对应于CA。10−10mol1− 1抗体。
Some aspects of improving surface plasmon resonance response in immunosensing applications are considered. Both from calculations and experiments, it was found that maximum sensitivity is obtained for a silver layer about 55 nm thick in direct contact with the species to be quantified. Application of an intermediate layer with high permittivity can be useful in suppressing background responses. Experimentally, a protein surface-coverage fraction of ca. 0.1 could be measured, corresponding to ca. 10−10mol1−1antibody.