Identification and analysis of alkaloids in cortex Phellodendron amurense by high-performance liquid chromatography with electrospray ionization mass spectrometry coupled with photodiode array detection
Identification and analysis of alkaloids in cortex Phellodendron amurense by high-performance liquid chromatography with electrospray ionization mass spectrometry coupled with photodiode array detection
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DOI：
10.1002/jssc.201400012
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发表时间：
2014-07-01
影响因子：
3.1
通讯作者：
Gao, Huiyuan
中科院分区：
文献类型：
--
作者：
Xian, Xiaoyan;Sun, Bohang;Gao, Huiyuan
黄柏中的生物碱采用高效液相色谱-电喷雾质谱联用技术（HPLC-ESI-MS），以XCharge C18柱为分离柱，采用光电二极管阵列检测器对生物碱进行定性和定量分析。通过在线ESI-MSn质谱裂解和紫外光谱分析，共鉴定出37种生物碱。首次从紫茎泽兰中分离鉴定出10种生物碱，其中4种为新的天然产物。分析了某些化合物的裂解途径。采用上述方法同时定量了一对异构体（Columbamine和药根碱）和四种主要生物碱（川楝素、木兰花碱、小檗碱和巴马汀）的含量。结果表明，新发现的和已知的苦玄参生物碱成分有助于确定苦玄参生物活性的物质基础，XCharge C-18柱是一种适合于植物生物碱分离的实用方法。
Alkaloids from Cortex Phellodendron amurense Rupr. were identified to determine the material basis for the bioactivity of this herb. HPLC-ESI-MS with photodiode array detection coupled to XCharge C18 column was applied to analyze the alkaloids qualitatively and quantitatively. A total of 37 alkaloids were identified and tentatively characterized from the ethanol extract by online ESI-MSn fragmentation and UV spectral analysis. A total of ten alkaloids, including four novel natural products, were tentatively identified for the first time in P. amurense. The fragmentation pathways for certain compounds were analyzed. The contents of a pair of isomers (columbamine and jatrorrhizine) and four main alkaloids (phellodendrine, magnoflorine, berberine, and palmatine) were simultaneously quantified using the aforementioned method. Results showed that the newly discovered and known components of P. amurense were helpful in determining the material basis for the bioactivity of the herb. The application of the XCharge C-18 column is a suitable and practical method for the isolation of alkaloids in plants.