Three dimensional microelectrode array device integrating multi-channel microfluidics to realize manipulation and characterization of enzyme-immobilized polystyrene beads
Three dimensional microelectrode array device integrating multi-channel microfluidics to realize manipulation and characterization of enzyme-immobilized polystyrene beads
复制标题
DOI：
10.1016/j.snb.2009.05.028
复制
发表时间：
2009-08
影响因子：
8.4
通讯作者：
Ryouta Kunikata;Yasufumi Takahashi;Masahiro Koide;T. Itayama;T. Yasukawa;H. Shiku;T. Matsue
中科院分区：
文献类型：
--
作者：
Ryouta Kunikata;Yasufumi Takahashi;Masahiro Koide;T. Itayama;T. Yasukawa;H. Shiku;T. Matsue
我们微制造了一种新颖的装置，由夹有两个微带电极阵列的 4×4 阵列微室组成。该装置通过在 DEP 力的基础上施加交流电压（10Vpp，10kHz），可以对微珠进行介电泳（DEP）操作，以引入和释放 V 形微室的特定地址。每个微室的两个电极（行电极和列电极）的设计和位置通过基于有限元方法的模拟进行了优化。更重要的是，电化学生成-收集测量可以评估酶活性。将固定有葡萄糖氧化酶(GOD)的微珠用DEP捕获在V形微室中后，引入含有3mM二茂铁甲醇(FcCH2OH)和0.1M葡萄糖的测量溶液。 V形微孔中的介质立即更换为测量溶液，而微珠留在微孔内而不施加DEP电压，因为微室内的流动与主通道的流动隔离。然后将行和列电极的电势设置为0.5和0.1V vs Ag/AgCl。 GOD 活性可以通过 [FcCH2OH]+ 还原电流的减少来监测。
We microfabricated a novel device consisting of a 4×4 array microchamber sandwiched with the two microband electrode array. This device allows dielectrophoretic (DEP) manipulation of microbeads to introduce into and release out a certain address of the V-shaped microchamber, by applying AC voltage (10Vpp, 10kHz) on the basis of DEP forces. The design and the position of the two electrodes (row and column electrodes) at each microchamber were optimized by simulation based on a finite element method. More importantly, electrochemical generation-collection measurement was possible to evaluate enzymatic activity. After microbeads immobilized with glucose oxidase (GOD) was entrapped in the V-shaped microchamber with DEP, a measuring solution containing 3mM ferrocenemethanol (FcCH2OH) and 0.1M glucose was introduced. The medium in the V-shaped microwell was immediately exchanged into the measuring solution whereas microbeads stayed within the microwell without applying DEP voltage, because the flow within the microchamber was isolated from that of the main channel. Then the potential of the row and column electrodes were set at 0.5 and 0.1V vs Ag/AgCl. The GOD activity can be monitored as the decrease in the [FcCH2OH]+reduction current.