Regulation of CCR6 chemokine receptor expression and responsiveness to macrophage inflammatory protein-3α/CCL20 in human B cells
Regulation of CCR6 chemokine receptor expression and responsiveness to macrophage inflammatory protein-3α/CCL20 in human B cells
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DOI：
10.1182/blood.v96.7.2338.h8002338_2338_2345
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发表时间：
2000-10-01
期刊：
影响因子：
20.3
通讯作者：
Richard, Y
中科院分区：
文献类型：
--
作者：
Krzysiek, R;Lefevre, EA;Richard, Y
分析了在B细胞个体发育和抗原驱动的B细胞分化期间CCR 6（趋化因子受体6）表达的调节。CD 34（+）Lin（-）造血干细胞祖细胞或CD 34（+）CD 19（+）（前B）或CD 19（+）CD 10（+）（前B/未成熟B细胞）B细胞祖细胞均不表达CCR 6。当CD 10丢失并且B细胞后代成熟时，获得CCR 6，进入表面免疫球蛋白D+（slgD（+））成熟B细胞库。CCR 6由所有骨髓、脐带血和外周血来源的幼稚和/或记忆B细胞表达，但不存在于次级淋巴器官的生发中心（GC）B细胞中。CCR 6在B细胞抗原受体触发后下调，并且在分化成分泌免疫球蛋白的浆细胞期间保持缺失，而在GC后记忆B细胞阶段重新获得。因此，在B细胞区室中，CCR 6的表达仅限于能够对抗原攻击作出反应的功能成熟细胞。在移行趋化试验中，巨噬细胞炎性蛋白（MIP）-3 α/CC趋化因子配体20（CCL 20）诱导B细胞剧烈移行，并对slgD（-）记忆B细胞具有不同的趋化偏好。这些数据表明，CCR 6表达和MIP-3 α/CCL 20反应性的限制模式是B系成熟和抗原驱动的B细胞分化过程的组成部分。（Blood.2000;96：2338-2345）（C）2000年，美国血液学会。
The regulation of CCR6 (chemokine receptor 6) expression during B-cell ontogeny and antigen-driven B-cell differentiation was analyzed. None of the CD34(+)Lin(-) hematopoietic stem cell progenitors or the CD34(+)CD19(+) (pro-B) or the CD19(+)CD10(+) (pre-B/immature B cells) B-cell progenitors expressed CCR6. CCR6 is acquired when CD10 is lost and B-cell progeny matures, entering into the surface immunoglobulin D+ (slgD(+)) mature B-cell pool. CCR6 is expressed by all bone marrow-, umbilical cord blood-, and peripheral blood-derived naive and/or memory B cells but is absent from germinal center (GC) B cells of secondary lymphoid organs. CCR6 is down-regulated after B-cell antigen receptor triggering and remains absent during differentiation into immunoglobulin-secreting plasma cells, whereas it is reacquired at the stage of post-GC memory B cells. Thus, within the B-cell compartment, CCR6 expression is restricted to functionally mature cells capable of responding to antigen challenge. In transmigration chemotactic assays, macrophage inflammatory protein (MIP)-3 alpha/CC chemokine ligand 20 (CCL20) induced vigorous migration of B cells with differential chemotactic preference toward slgD(-) memory B cells. These data suggest that restricted patterns of CCR6 expression and MIP-3 alpha/CCL20 responsiveness are integral parts of the process of B-lineage maturation and antigen-driven B-cell differentiation. (Blood.2000;96:2338-2345) (C) 2000 by The American Society of Hematology.