Detection of Babesia bovis using DNA hybridization.
Detection of Babesia bovis using DNA hybridization.
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DOI：
10.1111/j.1550-7408.1986.tb05571.x
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发表时间：
1986-02
期刊：
影响因子：
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通讯作者：
G. Mclaughlin;T. Edlind;G. Ihler
中科院分区：
文献类型：
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作者：
G. Mclaughlin;T. Edlind;G. Ihler
制备了含有牛巴氏杆菌DNA插入片段的质粒，并制备了适用于诊断B的克隆。牛感染被隔离。这些质粒可能含有重复序列，用这些质粒的DNA进行斑点杂交，过夜暴露后可检测到100 pg的B。牛DNA，其对应于50微升0.01%寄生虫血症红细胞中存在的DNA量。没有检测到的交叉杂交观察到与巴贝氏田鼠，恶性疟原虫，间日疟原虫，牛蜱，或牛的DNA。与10 ng二联巴氏杆菌DNA有少量交叉杂交。在杂交试验中使用这些探针可能有助于诊断牛和蜱中的巴贝斯虫病，确认菌株身份，并将毒力与特定的巴贝斯虫菌株相关联。
Plasmids containing inserts of Babesia bovis DNA were prepared and clones suitable for use in the diagnosis of B. bovis infections were isolated. Dot blot hybridization with DNA from these plasmids, which probably contain repetitive sequences, can detect after an overnight exposure 100 pg of B. bovis DNA, which corresponds to the amount of DNA present in 50 microliters of 0.01% parasitemic erythrocytes. No detectable cross-hybridization was observed with Babesia microti, Plasmodium falciparum, Plasmodium vivax, Boophilus, or cow DNA. A small amount of cross-hybridization was observed with 10 ng Babesia bigemina DNA. Use of these probes in a hybridization assay may be helpful in the diagnosis of babesiosis in cattle and ticks, in the confirmation of strain identities, and in correlating virulence with particular strains of Babesia.