The Anticonvulsant and Neuroprotective Effects of Baicalin on Pilocarpine-Induced Epileptic Model in Rats
The Anticonvulsant and Neuroprotective Effects of Baicalin on Pilocarpine-Induced Epileptic Model in Rats
复制标题
黄芩苷对毛果芸香碱致癫痫模型大鼠的抗惊厥和神经保护作用
DOI：
10.1007/s11064-012-0771-8
复制
发表时间：
2012-08-01
影响因子：
4.4
通讯作者：
Jiang, Wen
中科院分区：
文献类型：
--
作者：
Liu, Yang-Feng;Gao, Fei;Jiang, Wen
黄芩苷是从植物黄芩中提取的黄酮类化合物，具有抗氧化、抗凋亡和神经保护等多种药理活性。氧化应激可显著改变神经元功能，并与癫痫持续状态（SE）有关。然而，黄芩苷对癫痫的神经保护作用尚不清楚。本研究旨在探讨黄芩苷对匹罗卡品致痫大鼠的抗惊厥和神经保护作用。为此，我们记录了首次边缘系统发作和SE的潜伏期，并观察了SE的发生率和死亡率。在毛果芸香碱诱导SE后24 h测定氧化应激的变化。采用Nissl染色、末端脱氧核苷酸转移酶介导的dUTP缺口末端标记和Fluoro-Jade B染色检测匹罗卡品致痫后72 h海马神经元的丢失、凋亡和变性。黄芩苷预处理显著延迟首次边缘癫痫发作和SE的发生，降低死亡率，并减弱匹罗卡品处理大鼠海马中脂质过氧化、亚硝酸盐含量和还原型谷胱甘肽水平的变化。此外，我们还发现黄芩苷减轻了匹罗卡品诱导的大鼠海马神经元细胞丢失、凋亡和变性。总的来说，这些结果表明黄芩苷显着的抗惊厥和神经保护作用，并应鼓励进一步的研究，调查黄芩苷作为一种佐剂在癫痫发作，以防止癫痫发作，并防止癫痫发作引起的脑损伤。
Baicalin, a flavonoid compound purified from plant Scutellaria baicalensis Georgi, has been reported to possess a wide variety of pharmacological properties including anti-oxidative, anti-apoptotic and neuroprotective properties. Oxidative stress can dramatically alter neuronal function and has been linked to status epilepticus (SE). However, the neuroprotective effect of baicalin on epilepsy is unclear. In this study we investigated whether Baicalin could exert anticonvulsant and neuroprotective effects in the pilocarpine-induced epileptic model in rats. To this end, we recorded the latency to first limbic seizure and SE and observed the incidence of SE and mortality. The changes of oxidative stress were measured 24 h after pilocarpine-induced SE. Nissl staining, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling and Fluoro-Jade B staining were performed to detect the neuronal loss, apoptosis and degeneration in hippocampus 72 h after pilocarpine-induced seizure. Pretreatment with baicalin significantly delayed the onset of the first limbic seizures and SE, reduced the mortality rate, and attenuated the changes in the levels of lipid peroxidation, nitrite content and reduced glutathione in the hippocampus of pilocarpine-treated rats. Furthermore, we also found that baicalin attenuated the neuronal cell loss, apoptosis, and degeneration caused by pilocarpine-induced seizures in rat hippocampus. Collectively, these results indicated remarkable anticonvulsant and neuroprotective effects of baicalin and should encourage further studies to investigate baicalin as an adjuvant in epilepsy both to prevent seizures and to protect against seizure induced brain injury.