Pulsating electromagnetic field induces apoptosis of rat's bowel Cajal's cells.
Pulsating electromagnetic field induces apoptosis of rat's bowel Cajal's cells.
复制标题
DOI：
--
复制
发表时间：
2005
影响因子：
--
通讯作者：
J. Kaszuba-Zwoińska;A. Ziomber;K. Gil;A. Bugajski;W. Zaraska;P. Thor
中科院分区：
文献类型：
--
作者：
J. Kaszuba-Zwoińska;A. Ziomber;K. Gil;A. Bugajski;W. Zaraska;P. Thor
未标记我们之前已经证明脉动电磁场（PEMF）可以减少大鼠胃肠道中卡哈尔间质细胞（ICC）的表达。本研究的目的是确定是否减少表达的ICC在大鼠的肠PEMF暴露后与细胞凋亡和PEMF剂量。方法将大鼠分为两组（n= 32）。第一组（n = 16）暴露于四个剂量的PEMF，从一个剂量12.5 x 10（3）A（2）x h/m（2）到四个剂量50 x 10（3）A（2）x h/m（2）。第二组（n = 16）作为对照。将暴露于PEMF的大鼠和对照动物的十二指肠和结肠的组织样品固定并石蜡包埋，然后用低温恒温器冷冻。切向石蜡肠切片用抗c-Kit抗体染色。通过图像分析评估C-Kit阳性细胞。用兔多克隆抗Bax抗体检测大鼠组织细胞凋亡。结果PEMF刺激后，大鼠十二指肠和结肠c-Kit免疫阳性细胞的表面积呈剂量依赖性减少，促凋亡蛋白Bax的表达增加。PEMF对Cajal细胞的c-Kit免疫反应性的凋亡诱导作用表明与过度活跃的平滑肌功能障碍相关的疾病可能具有治疗意义。脉冲电磁场（PEMF）诱导大鼠肌间Cajal细胞免疫反应性改变。C-Kit降低ICCs的反应性被证明是由于PEMF刺激后ICCs中的凋亡途径被触发所致。PEMF诱导的细胞凋亡呈剂量依赖性，并通过免疫组化检测促凋亡蛋白Bax的表达。
UNLABELLED We previously have shown that pulsating electromagnetic field (PEMF) reduce expression of interstitial cells of Cajal (ICCs) in the rat gastrointestinal tract. Aim of present study was to determine whether diminished expression of ICCs in the rat's bowel after PEMF exposure was related to apoptosis and to PEMF dose. METHODS rats were divided into two groups (n= 32). First group (n = 16) was exposed to four rising doses of PEMF from one dose 12.5 x 10(3) A(2) x h/m(2) to four doses 50 x 10(3) A(2) x h/m(2). Second group (n = 16) served as a control. Tissue samples of the rat duodenum and colon from exposed to PEMF and control animals were fixed and paraffin embedded and cryostat frozen. The tangential paraffin bowel sections were stained with anti c-Kit antibody. C-Kit positive cells were assessed by image analysis. Apoptosis detection in rat's tissues was performed with rabbit polyclonal anti-Bax antibody. RESULTS the surface of c-Kit immunopositive cells decreased in the duodenum and colon of rats stimulated with PEMF in a dose dependent manner with increase in expression of pro-apoptotic Bax protein in c-Kit immunopositive myenteric cells. The apoptosis - inducing action of PEMF on the c-Kit immunoreactivity of Cajal's cells suggests a possible therapeutical implications in diseases associated with overactive smooth muscles dysfunction. Pulsating electromagnetic field (PEMF) induced changed immunoreactivity in rat's myenteric Cajal cells. C-Kit diminished reactivity of ICCs was proved to be caused by triggering of apoptotic pathwa in ICCs upon PEMF stimulation. PEMF generated apoptosis was dependant on applied dose of PEMF and detected by immunostaining with antibody against proapoptic protein Bax.