Effects of polyglycolic acid on porcine smooth muscle cell growth and differentiation
Effects of polyglycolic acid on porcine smooth muscle cell growth and differentiation
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DOI：
10.1002/jbm.a.10599
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发表时间：
2003-10-01
影响因子：
4.9
通讯作者：
Niklason, LE
中科院分区：
文献类型：
--
作者：
Higgins, SP;Solan, AK;Niklason, LE
聚乙醇酸（PGA）是常用的组织工程支架材料。最近的研究利用PGA作为支架血管组织工程使用牛和猪平滑肌细胞（SMCs）。在工程血管中，SMC在PGA片段存在的区域显示出高的有丝分裂率和去分化率。我们假设PGA分解产物在培养结束时被隔离在SMC血管内，导致血管SMC增殖和去分化增加。为了验证这一假设，本研究评估了PGA分解产物可能导致SMC表型变化的可能方法。SMC生长在高浓度的PGA分解产物显示，通过蛋白质印迹，降低钙调蛋白的表达，SMC分化的标志物。在乙醇酸（GA）中生长的SMC也是如此，其也显示增殖细胞核抗原（PCNA）（SMC增殖的标志物）的表达降低。相反，在不同量的NaCl或HCl中生长的细胞在分化方面几乎没有变化。我们的结论是，独立的酸度或渗透压，PGA降解的合理产品似乎诱导猪平滑肌细胞在体外去分化。由于去分化和有丝分裂减少，市售PGA可能不是血管组织工程的最佳支架。(C)2003 Wiley Periodicals，Inc.
Polyglycolic acid (PGA) is commonly used as a scaffold for tissue engineering. Recent studies utilized PGA as a scaffold for vascular tissue engineering using bovine and porcine smooth muscle cells (SMCs). In engineered vessels, the SMCs displayed high rates of mitosis and dedifferentiation in areas where PGA fragments were present. We hypothesized that PGA breakdown products, sequestered within a SMC vessel at the conclusion of culture, led to increased proliferation and dedifferentiation of vascular SMCs. To test this hypothesis, the current study assessed possible means by which PGA breakdown products could lead to changes in SMC phenotype. SMCs grown in high concentrations of PGA breakdown products showed, by Western blotting, decreased expression of calponin, a marker for SMC differentiation. The same was true for SMCs grown in glycolic acid (GA), which also showed decreased expression of proliferating cell nuclear antigen (PCNA), a marker for SMC proliferation. In contrast cells grown in varying amounts of NaCl or HCl showed little change in differentiation. We conclude that, independent of acidity or osmolality, plausible products of PGA degradation appear to induce dedifferentiation of porcine SMCs in vitro. Because of dedifferentiation and decreased mitosis, commercially available PGA may not represent an optimal scaffold for vascular tissue engineering. (C) 2003 Wiley Periodicals, Inc.