Human erythema and matrix metalloproteinase-1 mRNA induction, in vivo, share an action spectrum which suggests common chromophores
Human erythema and matrix metalloproteinase-1 mRNA induction, in vivo, share an action spectrum which suggests common chromophores
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体内人红斑和基质金属蛋白酶 1 mRNA 诱导具有共同的作用谱，表明存在共同的发色团
DOI：
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发表时间：
2012
影响因子：
3.1
通讯作者：
A. Young
中科院分区：
文献类型：
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作者：
A. Tewari;Christine Lahmann;R. Sarkany;J¨org Bergemann;A. Young
基质金属蛋白酶1（MMP-1）被广泛认为是光老化的生物标志物。我们测试的假设，MMP-1 mRNA的表达和红斑共享一个共同的行动频谱，通过比较的影响，在整个人体皮肤急性MMP-1 mRNA的表达在体内的紫外线等效剂量，UVA 1和太阳模拟辐射（SSR）。我们的研究结果表明，MMP-1的表达与所有三个光谱，这支持了我们的假设。作用光谱的共享意味着共同的发色团，其中之一可能是DNA。我们以前已经表明，我们使用的所有光谱容易诱导环丁烷胸腺嘧啶二聚体（T<>T）在人体表皮在体内，但我们缺乏定量的数据对真皮DNA的损伤。这一点很重要，因为我们不知道真皮MMP-1诱导是通过对真皮的直接损伤还是通过对表皮的间接损伤发生的。我们的研究结果表明，UVB诱导的T<>T约3倍，与皮肤等效剂量的UVA 1相比，这与我们发表的表皮数据相似。这支持了以前发表的工作，也涉及一个未知的UVA 1发色团的红斑和MMP-1诱导。然而，真皮DNA损伤的分布随光谱变化很大。在UVB的情况下，它主要是在上层真皮，但与UVA 1，它是均匀分布的。因此，无论发色团，MMP-1诱导直接皮肤损伤的光谱是可能的。我们的数据的实际结论是，太阳紫外线辐射的UVB含量很小（<5%）可能是光老化的主要原因，至少在MMP-1表达方面。此外，通过使用防晒霜预防红斑可能导致MMP-1表达减少。
Matrix metalloproteinase 1 (MMP-1) is widely regarded as a biomarker of photoageing. We tested the hypothesis that MMP-1 mRNA expression and erythema share a common action spectrum by comparing the effects of erythemally equivalent doses of UVB, UVA1 and solar simulated radiation (SSR) on acute MMP-1 mRNA expression in whole human skin in vivo . Our results show comparable MMP-1 expression with all three spectra, which supports our hypothesis. The sharing of an action spectrum implies common chromophores, one of which is likely to be DNA. We have previously shown that all spectra that we used readily induce cyclobutane thymine dimers (T<>T) in human epidermis in vivo but we lack quantitative data on damage to dermal DNA. This is important because we do not know if dermal MMP-1 induction occurs via direct damage to the dermis, or indirectly via damage to the epidermis. Our results show that UVB induces about 3 times more T<>T compared with erythemally equivalent doses of UVA1, which is similar to our published epidermal data. This supports previously published work that also implicates an unknown UVA1 chromophore for erythema and MMP-1 induction. However, the distribution of the dermal DNA damage varies considerably with spectrum. In the case of UVB it is primarily in the upper dermis, but with UVA1 it is evenly distributed. Thus, irrespective of chromophores, MMP-1 induction by direct dermal damage by both spectra is possible. The practical conclusions of our data are that the small (<5%) UVB content of solar UVR is likely to be the main cause of photoageing, at least in terms of MMP-1 expression. Furthermore, prevention of erythema by sunscreen use is likely to result in reduced MMP-1 expression.
影响因子：
3.6
作者：
Lockwood, Charles J.;Oner, Ceyda;Schatz, Frederick
通讯作者：
Schatz, Frederick