Performance of mice in an automated olfactometer: Odor detection, discrimination and odor memory
Performance of mice in an automated olfactometer: Odor detection, discrimination and odor memory
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DOI：
10.1093/chemse/24.6.637
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发表时间：
1999-12-01
期刊：
影响因子：
3.5
通讯作者：
Slotnick, B
中科院分区：
文献类型：
--
作者：
Bodyak, N;Slotnick, B
在100或200次试验中，使用去，不去离散试验操作性条件反射程序对小鼠进行各种气味检测和辨别训练。气味，在每次试验1秒，产生的空气稀释嗅觉仪（阈值测试）和一个容易构造的八通道液体稀释单元（两个和多个气味辨别任务）。小鼠迅速获得操作任务，并表现出良好的刺激控制气味蒸汽。其乙酸乙酯的绝对检测阈值与使用类似方法从大鼠中获得的结果相似。他们很容易获得四个独立的两种气味辨别任务，并继续表现良好时，所有八种气味以随机顺序在同一会话中，当正确反应的强化概率从1下降到0.5。这八种气味的记忆，在3 t天的休息期后在消退下评估，基本上是完美的。在S+和S-试验上采样气味所花费的时间与响应准确性高度相关。当准确性处于偶然水平时（例如，对新任务的初始试验），S+和S-试验上的刺激采样时间类似于0.5-0.7 s。随着响应准确度的增加，S+试验的采样时间趋于增加，并保持高于S-试验的采样时间。
Mice were trained on a variety of odor detection and discrimination lash in 100- or 200-trial sessions using a go, no-go discrete trials operant conditioning procedure. Odors, presented for 1 s on each trial, were generated by an air dilution olfactometer (for threshold tests) and an easily constructed eight-channel liquid dilution unit (for two- and multiple-odor discrimination tasks). Mice rapidly acquired the operant task and demonstrated excellent stimulus control by odor vapors. Their absolute detection threshold for ethyl acetate was similar to that obtained with rats using similar methods. They readily acquired four separate two-odor discrimination tasks and continued to perform well when all eight odors were presented in random order in the same session and when reinforcement probability for correct responding was decreased from 1 to 0.5. Memory for these eight odors, assessed under extinction after a 3t day rest period, was essentially perfect. Time spent sampling the odor on S+ and S- trials was highly correlated with response accuracy When accuracy was at chance levels (e.g. initial trials on a novel task), stimulus sampling time on both S+ and S- trials was similar to 0.5-0.7 s. As response accuracy increased, sampling time on S+ trials tended to increase and remain higher than sampling time on S- trials.