ISOLATION AND CHARACTERIZATION OF MACROVASCULAR AND MICROVASCULAR ENDOTHELIAL-CELLS FROM HUMAN HEARTS
ISOLATION AND CHARACTERIZATION OF MACROVASCULAR AND MICROVASCULAR ENDOTHELIAL-CELLS FROM HUMAN HEARTS
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DOI：
10.1152/ajpheart.1994.267.6.h2138
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发表时间：
1994-12-01
影响因子：
4.8
通讯作者：
FLECK, E
中科院分区：
文献类型：
--
作者：
GRAFE, M;AUCHSCHWELK, W;FLECK, E
目前还没有研究大血管和微血管冠状动脉循环中局部止血机制的体内模型。因此，我们建立了一个高细胞产量和高内皮细胞纯度的人大血管和微血管内皮细胞培养体系。通过酶处理心脏移植过程中获得的心肌制备物，从人心脏分离微血管内皮细胞。分离的微容器用于启动培养物，随后通过与凝集素荆豆凝集素I连接的顺磁珠从污染的非内皮细胞中分离和纯化。从心外膜冠状动脉分离大血管内皮细胞，并通过顺磁珠纯化。此方法纯度高（
In vivo models to investigate mechanisms of local hemostasis in the macro- and microvascular coronary circulation are not available. Therefore, we established a culture system of human macro- and microvascular endothelial cells with high cellular yield and high endothelial cell purity. Microvascular endothelial cells from human hearts were isolated by enzymatic treatment of cardiac muscle preparations obtained during heart transplantation. The isolated microvessels were used to start cultures that were subsequently separated and purified from contaminating nonendothelial cells by paramagnetic beads linked to the lectin Ulex europaeus agglutinin I. Macrovascular endothelial cells were isolated from epicardial coronary arteries and purified by paramagnetic beads as well. With this method high purity (