Conserved neural circuit structure across Drosophila larval development revealed by comparative connectomics
Conserved neural circuit structure across Drosophila larval development revealed by comparative connectomics
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DOI：
10.7554/elife.29089
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发表时间：
2017-10-23
期刊：
影响因子：
7.7
通讯作者：
Schneider-Mizell, Casey M.
中科院分区：
文献类型：
--
作者：
Gerhard, Stephan;Andrade, Ingrid;Schneider-Mizell, Casey M.
在胚胎后期发育期间，神经系统必须适应成长中的身体。神经元结构和连接性的改变如何有助于维持适当的电路功能仍不清楚。此前，我们测量了果蝇突触连接背后的细胞神经解剖学(Schneider-Mizell等人，2016)。在这里，我们用连续切片电子显微镜研究了一龄幼虫和三龄幼虫之间神经元形态和连通性的变化。我们在每个阶段的幼虫中重建了伤害性感受电路，并发现识别出的神经元之间在拓扑上排列一致的连接性。每个突触的大小、终末树突分支的数量和突触输入的总数增加了五倍，伴随着细胞类型特定的连接性变化，保留了与每个突触前伙伴相关的总突触输入的比例。我们建议，结构增长的精确模式可以保存电路的计算功能，例如确定危险刺激的位置。
During postembryonic development, the nervous system must adapt to a growing body. How changes in neuronal structure and connectivity contribute to the maintenance of appropriate circuit function remains unclear. Previously, we measured the cellular neuroanatomy underlying synaptic connectivity in Drosophila (Schneider-Mizell et al., 2016). Here, we examined how neuronal morphology and connectivity change between first instar and third instar larval stages using serial section electron microscopy. We reconstructed nociceptive circuits in a larva of each stage and found consistent topographically arranged connectivity between identified neurons. Five-fold increases in each size, number of terminal dendritic branches, and total number of synaptic inputs were accompanied by cell type-specific connectivity changes that preserved the fraction of total synaptic input associated with each pre-synaptic partner. We propose that precise patterns of structural growth act to conserve the computational function of a circuit, for example determining the location of a dangerous stimulus.