Detection of Hypoxia-Induced and Iron Depletion-Induced Mitophagy in Mammalian Cells.
Detection of Hypoxia-Induced and Iron Depletion-Induced Mitophagy in Mammalian Cells.
复制标题
哺乳动物细胞中缺氧诱导和缺铁诱导的线粒体自噬的检测。
DOI：
10.1007/7651_2017_19
复制
发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Kanki T.
中科院分区：
文献类型：
--
作者：
Yamashita SI;Kanki T.
线粒体的质量和数量不仅受线粒体融合和分裂的影响，而且受线粒体降解的影响。有丝分裂是一种针对受损或不必要线粒体的自噬，被认为是线粒体动态平衡的重要途径。到目前为止，已知有几种刺激可以诱导有丝分裂。然而，其中一些刺激，包括缺氧、铁耗竭和氮饥饿，会诱导轻微的有丝分裂，这种现象很难通过线粒体质量的下降来检测到。最近，我们利用Mito-Keima作为记者，清楚地检测到了在这些条件下诱导的有丝分裂。在这一章中，我们描述了使用mito-keima表达的细胞来诱导和检测缺氧诱导和铁耗竭诱导的有丝分裂吞噬的方案。
Mitochondrial quality and quantity are not only regulated by mitochondrial fusion and fission but also by mitochondria degradation. Mitophagy, an autophagy specific for damaged or unnecessary mitochondria, is believed to be an important pathway for mitochondrial homeostasis. To date, several stimuli are known to induce mitophagy. Some of these stimuli, however, including hypoxia, iron depletion, and nitrogen starvation, induce mild mitophagy, which is difficult to detect through decreased mitochondrial mass. Recently, we have clearly detected mitophagy induced under these conditions using mito-Keima as a reporter. In this chapter, we describe the protocols for induction and detection of hypoxia-induced and iron depletion-induced mitophagy using mito-Keima-expressed cells.