Improving Chronic Diabetic Wound Healing through an Injectable and Self-Healing Hydrogel with Platelet-Rich Plasma Release
Improving Chronic Diabetic Wound Healing through an Injectable and Self-Healing Hydrogel with Platelet-Rich Plasma Release
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通过释放富含血小板的血浆的可注射自愈水凝胶改善慢性糖尿病伤口愈合
DOI：
10.1021/acsami.0c17142
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发表时间：
2020-12-16
影响因子：
9.5
通讯作者：
Liu, Haifeng
中科院分区：
文献类型：
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作者：
Qian, Zhiyong;Wang, Haiping;Liu, Haifeng
糖尿病皮肤溃疡是糖尿病的严重并发症之一，发病率高，可导致死亡或致残。富血小板血浆（Platelet-rich plasma，PRP）因其所含的生长因子（growth factor，GFs）而被广泛应用于糖尿病创面的治疗，但其半衰期较短限制了其临床应用。此外，糖尿病伤口微环境中大量蛋白酶的存在导致GF降解，这进一步阻碍血管生成和糖尿病伤口愈合。在我们的研究中，我们制造了一种具有自愈性和可注射性的水凝胶，其具有壳聚糖、丝素蛋白和PRP的复合物（CBPGCTS-SF@PRP），用于促进糖尿病伤口愈合。CBPGCTS-SF@PRP可保护PRP免受酶解，持续释放PRP，增强间充质干细胞的趋化性。结果表明，它能促进修复细胞的体外增殖。此外，在2型糖尿病大鼠模型和大鼠皮肤缺损模型中，它可以通过加速胶原沉积、血管生成和神经修复来促进伤口愈合。希望本研究能为临床糖尿病不愈合创面的治疗提供新的思路。
Diabetic skin ulcer is one of the severe complications of diabetes mellitus, which has a high incidence and may cause death or disability. Platelet-rich plasma (PRP) is widely used in the treatment of diabetic wounds due to the effect of growth factors (GFs) derived from it. However, the relatively short half-life of GFs limits their applications in clinics. In addition, the presence of a large amount of proteases in the diabetic wound microenvironment results in the degradation of GFs, which further impedes angiogenesis and diabetic wound healing. In our study, we fabricated a self-healing and injectable hydrogel with a composite of chitosan, silk fibroin, and PRP (CBPGCTS-SF@PRP) for promoting diabetic wound healing. CBPGCTS-SF@PRP could protect PRP from enzymatic hydrolysis, release PRP sustainably, and enhance the chemotaxis of mesenchymal stem cells. The results showed that it could promote the proliferation of repair cells in vitro. Moreover, it could enhance wound healing by expediting collagen deposition, angiogenesis, and nerve repair in a type 2 diabetic rat model and a rat skin defect model. We hope that this study will offer a new treatment for diabetic nonhealing wounds in clinics.