Differential expression of NeuroD in primary cultures of cerebral cortical neurons
Differential expression of NeuroD in primary cultures of cerebral cortical neurons
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DOI：
10.1006/excr.1997.3757
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发表时间：
1997-11-01
影响因子：
3.7
通讯作者：
Ishii, K
中科院分区：
文献类型：
--
作者：
Katayama, M;Mizuta, I;Ishii, K
我们已经研究了一个基本的螺旋-环-螺旋调节基因，neuroD，在小鼠大脑皮层神经元的原代培养的表达模式，在原代培养的神经元的分化状态，确定由神经元的敏感性谷氨酸毒性和特定的蛋白质，如磷酸化形式的200 kDa的神经丝，HPC-1/syntaxin 1A，和细胞粘附分子L1的表达。采用RT-PCR和原位杂交技术检测neuroD的表达。由此获得的实验结果显示，如已知的，神经元成熟在培养的第7天和第11天之间开始，并且neuroD的表达随着培养天数的增加而降低。基于这些发现，可以得出结论，neuroD在未成熟的神经元中表达，但在成熟的神经元中不表达。(C)北京：科学出版社.
We have investigated the expression patterns of a basic helix-loop-helix regulatory gene, neuroD, in primary cultures of murine cerebral cortical neurons, The differentiation states of neurons in primary cultures were determined by the sensitivity of neurons to glutamate toxicity and the expression of specific proteins such as the phosphorylated form of a 200-kDa neurofilament, HPC-1/syntaxin 1A, and cell adhesion molecule L1. The expression of neuroD was determined by RT-PCR analysis and in situ hybridization. The experimental results thus obtained revealed that neuronal maturation is initiated between Day 7 and Day 11 in the culture as already known, and that the expression of neuroD decreases with increasing days in culture. Based on these findings, it was concluded that neuroD is expressed in immature neurons but not in mature ones. (C) 1997 Academic Press.